产品简要
公司名称 :
LifeSpan Biosciences
产品类型 :
抗体
产品名称 :
肿瘤坏死因子甲抗体LS-C104683
目录 :
LS-C104683
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定
更多信息或购买 :
产品信息
抗体编号 :
106533
抗体名称 :
LS-C104683
目标物种 :
人类
宿主物种 :
小鼠
产品名称 :
肿瘤坏死因子甲抗体LS-C104683
特异性 :
人类肿瘤坏死因子甲
克隆描述 :
单克隆
抗体修饰 :
未共轭
样品状态 :
冻干来自PBS
免疫原类型 :
细胞提取物
免疫原描述 :
E.coli derived recombinant Human TNF-alpha.
纯化描述 :
离子交换层析
推荐的储存条件 :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
重悬液体 :
Reconstitute in sterile water to 1 mg/ml.
抗体亚型 :
IgG1
基因 :
肿瘤坏死因子甲
标准基因符号 :
肿瘤坏死因子
家族 :
肿瘤坏死因子
反应物种 :
人类
用途 :
酶联免疫吸附测定(2-4微克/毫升), 免疫印迹(0.5-1微克/毫升)
ShortWebDescription :
TNF Alpha antibody LS-C104683 is an unconjugated mouse monoclonal antibody to human TNF Alpha. Validated for ELISA and WB.
使用文本 :
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 15-20 ug/ml overnight at 4°C. An HRP-labeled polymer detection system was used with DAB Chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. *Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-alpha (0.5 ng/ml), a concentration of < 0.1 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human TNF-alpha when used with biotinylated antigen affinity purified anti-Human TNF-alpha (LS-C104719) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect Human TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.5-1 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TNF-alpha is 0.5-1 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
同义词 :
TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2
SalesRegion :
Worldwide
更多信息或购买 :
公司信息
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
公司总部: 美国
LifeSpan成立于1995年,已经成为分子病理学领域的领导品牌,专注于人和其他物种的正常和疾病组织中蛋白的定位情况。我们提供超过74000种抗体,定制的免疫组化研究服务,超过500种蛋白研究用人免疫组化材料,及组化和病理学服务;提供2,000,000样本,包括冰冻及石蜡切片的正常和疾病组织,进行对外研究服务。对外服务项目范围广泛,由LifeSpan的病理学家提供包括课题设计、抗体搜寻及表征、组织搜集和验证、免疫标记、问题处理及结果处理等诸多环节。