抗体

TP53/p53抗体(aa20-25, 克隆DO-7)LS-C11556

LS-C11556

单克隆

小鼠

未共轭

DO-7

免疫印迹, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片

LS-C11556

人类

小鼠

TP53/p53抗体(aa20-25, 克隆DO-7)LS-C11556

Recognizes the human 53kD p53 tumor suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramerization domain and a terminal regulatory domain. p53 is intimately involved in a number of signaling pathways controlling cell division, cycling and apoptosis and is thus a potent cancer suppressor. In normal cells the level of p53 expression is low but can be induced by DNA damage or other stress signals. Activation of p53 leads to growth arrest through its interaction with p21, GADD45 and, DNA repair and potentially apoptosis through interaction with,, and. p53 is critically regulated by Mdm2 which can trigger p53 degradation by a ubiquitin dependent systemrecognizes an epitope at the N-terminal end of p53 between amino acids 20-25, binding to both wild type and mutant forms. Clone DO-7 is not expected to recognize the multiple lacking the N-terminal region.

单克隆

DO-7

未共轭

aa20-25

PBS, 0.09% 叠氮化钠

重组蛋白

重组人类p53(氨基酸20-25)

纯化IgG

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

IgG2b

TP53/p53

TP53

P53

人类

小鼠, 大鼠

Flo (1:50 - 1:100), IHC-Fr, IHC-P (1:1000), IP, WB (1:1000)

P53 antibody LS-C11556 is an unconjugated mouse monoclonal antibody to human p53 (TP53) (aa20-25). Validated for Flow, IHC, IP and WB.

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 whole blood in 100ul. Membrane permeabilization is required for this application. Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Histology Positive Control Tissue: Colon or breast carcinoma.

TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1

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