抗体

AKT1抗体(aa2-480)LS-C125031

LS-C125031

单克隆

小鼠

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪

LS-C125031

人类

小鼠

AKT1抗体(aa2-480)LS-C125031

Recognizes human Akt1. Does not detect rhAkt2 or rhAkt3.

单克隆

未共轭

aa2-480

Lyophilized from Sterile Solution PBS, 5% Trehalose

Sf 21-derived recombinant human v-akt murine thymoma viral oncogene homolog 1 aa2-480.

蛋白质G纯化

Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

Reconstitute in sterile PBS.

IgG1

AKT1

AKT1

蛋白激酶

RAC/AKT

人类

ELISA, Flo (50 µg/ml), ICC (8 - 25 µg/ml), WB

AKT1 antibody LS-C125031 is an unconjugated mouse monoclonal antibody to human AKT1 (aa2-480). Validated for ELISA, Flow, ICC and WB.

Suitable for use in ELISA, Western Blot, Immunocytochemistry, Suitable for use in Flow cytometry: 50 ug/ml. This antibody was validated for flow cytometry using MCF-7 cells. For. intracellular staining to detect Akt1, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-5x10^5 cells in a total reaction volume not exceeding 20ulL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis. Immunocytochemistry: 8-25 ug/ml to detect Akt1 in fixed human peripheral blood mononuclear cells.

AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA

Worldwide