抗体

AKT1抗体(内部, 克隆14E5.16C8.25F6)LS-C154001

LS-C154001

单克隆

小鼠

未共轭

14E5.16C8.25F6

免疫印迹, 酶联免疫吸附测定, 免疫组化, 流式细胞仪

LS-C154001

人类

小鼠

AKT1抗体(内部, 克隆14E5.16C8.25F6)LS-C154001

This antibody is specific for human and mouse AKT protein. A BLAST analysis was used to suggest cross-reactivity with AKT1, AKT2, and AKT3 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT1, 2, 3 was determined with Western Blot. Cross reactivity of AKT from other sources has not been determined.

单克隆

14E5.16C8.25F6

未共轭

内部

0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠, 0.01% 叠氮化钠

合成肽

Anti-AKT Antibody was produced by repeated immunizations in mice with a synthetic peptide corresponding to residues internal to the human AKT1 protein.

蛋白质A亲和色谱

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

IgG

AKT1

AKT1

蛋白激酶

RAC/AKT

小鼠, 大鼠, 黑猩猩, 人类

ELISA (1:20000), Flo, IHC (20 µg/ml), WB (1:500 - 1:3000)

AKT1 antibody LS-C154001 is an unconjugated mouse monoclonal antibody to AKT1 (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC and WB.

Anti-AKT Antibody is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kD in size corresponding to AKT protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human and mouse AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative.

AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA

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