NCL Antibody (Center E443) immunohistochemistry of formalin-fixed and paraffin-embedded human breast tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Fluorescent confocal image of HeLa cell stained with NCL Antibody (Center E443). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NCL immunoreactivity is localized to nucleus significantly.