抗体

MAPK8/JNK1抗体LS-C17753

LS-C17753

多克隆

domestic rabbit

未共轭

人类, 大鼠

免疫印迹, 免疫沉淀

LS-C17753

大鼠

兔

MAPK8/JNK1抗体LS-C17753

Recognizes SAPK1a (54kD). Weakly recognizes SAPK1b (54kD). The protein detected with a MWapp of 45kD may be SAPK1c. Species Reactivity: Human, mouse and rat.

多克隆

未共轭

0.01 M三羟甲基氨基甲烷甘氨酸, pH值7.4, 0.15 M氯化钠, 0.05% 叠氮化钠, 30% 甘油

融合蛋白

GST fusion protein corresponding to full-length rat SAPK1a. This sequence is approximately 85% identical to rat SAPK1b and rat SAPK1c. These sequences are highly conserved among mammalian species.

蛋白质A纯化

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

IgG

MAPK8/JNK1

MAPK8

蛋白激酶

MAPK

小鼠, 大鼠, 人类

Func, IP, WB

JNK1 antibody LS-C17753 is an unconjugated rabbit polyclonal antibody to JNK1 (MAPK8) from rat. It is reactive with human, mouse and rat. Validated for Func, IP and WB.

Suitable for use in Western Blot analysis and Immunoprecipitation. Western Blot Analysis: 1 ug/ml detected SAPK1 in RIPA lysates from human A431, murine 3T3 and rat PC-12 cells. At 1 ug/ml, the signal detected with 10 ng of gst-SAPK1a was approximately three times greater than the signal detected of with 10 ng gst-SAPK1b. Included Positive Antigen Control: Non-stimulated A431 cell lysate. Use 20 ug per lane for minigels. Immunoprecipitation Protein Kinase Assay: 4 ug immunoprecipitated active recombinant rat SAPK1a, which then catalyzed the transfer of the y-phosphate of [y-32 P]-ATP to c-Jun protein substrate. Western Blot Analysis A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SAPK1 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

MAPK8, JNK, JNK1, JNK1A2, JNK-46, 地图激酶8, PRKM8, SAPK1, SAPK1c, MAPK 8, c Jun氨基末端激酶1, JNK21B1/2, JUN N末端激酶

Worldwide