产品简要
公司名称 :
LifeSpan Biosciences
产品类型 :
抗体
产品名称 :
CDK1/CDC2抗体(克隆POH-1)LS-C18772
目录 :
LS-C18772
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
POH-1
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 3
| 参考文献 |
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Palmer A, Gavin A, Nebreda A. A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1. EMBO J. 1998;17:5037-47 pubmed
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Ye X, Fincher R, Tang A, McNeal K, Gygax S, Wexler A, et al. Proteolysis and tyrosine phosphorylation of p34cdc2/cyclin B. The role of MCM2 and initiation of DNA replication to allow tyrosine phosphorylation of p34cdc2. J Biol Chem. 1997;272:33384-93 pubmed
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Lukas J, Draetta G, Bartek J. Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur J Biochem. 1992;207:169-76 pubmed
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产品信息
抗体编号 :
19520
抗体名称 :
LS-C18772
目标物种 :
人类
宿主物种 :
小鼠
产品名称 :
CDK1/CDC2抗体(克隆POH-1)LS-C18772
特异性 :
Reacts specifically with p34 cdc2 in human tissues and cell lines. This antibody is not cross reactive with other cyclin dependent kinases. Cross reactivity with p34 cdc2 from other sources, especially mouse and rat will occur. This reagent has broad interspecies reactivity.
克隆描述 :
单克隆
克隆名称 :
POH-1
抗体修饰 :
未共轭
样品状态 :
0.02 M钾磷酸盐, pH值7.2, 0.5 M氯化钠, 0.01% 叠氮化钠
免疫原类型 :
重组蛋白
免疫原描述 :
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.
纯化描述 :
蛋白质A纯化
推荐的储存条件 :
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
抗体亚型 :
IgG2a, k
基因 :
CDK1/CDC2
标准基因符号 :
CDK1
家族 :
蛋白激酶
亚家族 :
CDC2/CDK
反应物种 :
小鼠, 大鼠, 人类
用途 :
酶联免疫吸附测定(1:5000 - 1:20000), 免疫组化, 免疫组化-P, 免疫沉淀(1:100), 免疫印迹(1:500 - 1:1000)
ShortWebDescription :
CDC2 antibody LS-C18772 is an unconjugated mouse monoclonal antibody to CDC2 (CDK1) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC, IP and WB. Cited in 3 publications.
使用文本 :
This antibody is suitable for immunohistochemistry immunoprecipitation (as active kinase), and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products. HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting. LEP fibroblast cell lysate is suggested as a negative control. Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1 % saponin treatment or other permeabilization method.
同义词 :
CDK1, CDC28A, CDC2, CDKN1, Cyclin-dependent kinase 1, p34 protein kinase, p34CDC2, CDC2a, Cell cycle controller CDC2, Cell division protein kinase 1, PSTAIR
SalesRegion :
Worldwide
更多信息或购买 :
公司信息
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com1-206-464-1554
公司总部: 美国
LifeSpan成立于1995年，已经成为分子病理学领域的领导品牌，专注于人和其他物种的正常和疾病组织中蛋白的定位情况。我们提供超过74000种抗体，定制的免疫组化研究服务，超过500种蛋白研究用人免疫组化材料，及组化和病理学服务；提供2,000,000样本，包括冰冻及石蜡切片的正常和疾病组织，进行对外研究服务。对外服务项目范围广泛，由LifeSpan的病理学家提供包括课题设计、抗体搜寻及表征、组织搜集和验证、免疫标记、问题处理及结果处理等诸多环节。