抗体

表皮生长因子受体抗体(磷酸化Tyr1197)LS-C18796

LS-C18796

多克隆

domestic rabbit

未共轭

磷酸化

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片

LS-C18796

人类

兔

表皮生长因子受体抗体(磷酸化Tyr1197)LS-C18796

Reactivity occurs against human EGFR pY1197 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human EGFR is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with phosphorylated EGFR from human, mouse and rat sources. Reactivity of this antibody with EGFR from other species is unknown.

多克隆

未共轭

pTyr1197

0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠, 0.01% 叠氮化钠

合成肽

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminus near amino acids 1175-1200 of human EGFR protein.

亲和纯化

Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

IgG

表皮生长因子受体

表皮生长因子受体

蛋白激酶

EGF受体

人类

酶联免疫吸附测定(1:4000 - 1:20000), 免疫组化, 免疫组化-P(5微克/毫升), 免疫印迹(1:250 - 1:1500)

EGFR antibody LS-C18796 is an unconjugated rabbit polyclonal antibody to human EGFR (pTyr1197). Validated for ELISA, IHC and WB.

This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 170 kD in size corresponding to phosphorylated EGFR protein by western blotting in the appropriate cell lysate or extract. Less than 5.0% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pY1197 of EGFR protein.

EGFR, EGF Receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1

Worldwide