抗体

胰岛素受体底物1抗体(磷酸化Ser307)LS-C18824

LS-C18824

多克隆

domestic rabbit

未共轭

磷酸化

免疫印迹, 酶联免疫吸附测定, 免疫组化

LS-C18824

人类

兔

胰岛素受体底物1抗体(磷酸化Ser307)LS-C18824

Reactivity occurs against human IRS1 pS307 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human IRS1 is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with IRS1 from human, mouse, rat, dog and vervet monkey based on 100% homology with the immunizing sequence. Partial reactivity is also expected against IRS1 from pig (94%), bovine (94%) and chicken (88%) sources. Reactivity of this antibody with IRS1 from other species is unknown.

多克隆

未共轭

pSer307

0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠, 0.01% 叠氮化钠

合成肽

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 290-320 of human IRS1 protein.

亲和纯化

Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

IgG

胰岛素受体底物1

胰岛素受体底物1

人类

酶联免疫吸附测定(1:2000 - 1:10000), 免疫组化, 免疫印迹(1:250 - 1:1500)

IRS1 antibody LS-C18824 is an unconjugated rabbit polyclonal antibody to human IRS1 (pSer307). Validated for ELISA, IHC and WB.

This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 180 kD in size corresponding to phosphorylated IRS1 protein by western blotting in the appropriate cell lysate or extract. Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS307 of IRS1 protein.

IRS1, IRS-1, HIRS-1, Insulin receptor substrate 1

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