抗体

IL-1B/IL-1乙抗体(N末端)LS-C18827

LS-C18827

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 中和反应, 免疫沉淀, 流式细胞仪, 放射免疫分析法, 免疫组化-石蜡切片, 免疫组化-冰冻切片

LS-C18827

人类

兔

IL-1B/IL-1乙抗体(N末端)LS-C18827

This antibody is primarily directed against mature, 17,000 MW human IL-1ß and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1ß in the same formats using similar dilutions. The antiserum does not recognize human IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW IL-1ß containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1ß precursor molecule. Denatured 31,000 precursor IL-1ß will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1ß exposing epitopes not exposed in the natural state. In immunoblots, depending on the number of cells, the antibody detects the 17,000 MW band in supernatants as well as a 35,000 MW band representing the 31,000 MW IL-1ß precursor in lysates.

多克隆

未共轭

N末端

0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠

重组蛋白

This antibody was prepared by repeated immunizations with recombinant human IL-1ß produced in E.coli.  The MW of the recombinant 153 aa IL-1ß was 17 kDa with the N-terminal amino acid at position alanine 117.  This cleavage site is generated by the IL-1ß converting enzyme (ICE, capase-1).

DEAE fractionation

分成小份儿保存在-20°C。 避免冻融循环

IgG

IL-1B/IL-1乙

IL1B

IL-1

狗, 人类, 灵长类动物

ELISA (1:500 - 1:2000), Flo, IF, IHC, IHC-Fr (1:100 - 1:200), IHC-P (1:100 - 1:200), IP (1:400 - 1:800), Neut (1:100), RIA (1:5000 - 1:10000), WB (1:1000)

IL-1 Beta antibody LS-C18827 is an unconjugated rabbit polyclonal antibody to IL-1 Beta (IL-1B) (N-Terminus) from human. It is reactive with human, dog and primate. Validated for ELISA, Flow, IF, IHC, IP, Neut, RIA and WB. Cited in 2 publications.

This IgG fraction antibody of anti- Human IL-1beta has been tested for use in neutralizations, ELISA, radioimmunoassays, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17000 MW mature IL-10. For immunoblots, typically, IL-1beta is detected from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coli LPS. For immunoprecipitation a dilution of 1:400 to 1:800 is suggested. Pre-clearing the preparation with a non-specific rabbit IgG (011-001-297) to reduce background. For immunohistochemistry a dilution of 1:100 to 1:200 is suggested. Either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL- 1beta. For ELISA also use HRP Conjugated Anti-Rabbit IgG [HalphaL] (Goat) (611-1302) for detection.

IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta

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