抗体

胶原II抗体(生物素)LS-C18866

LS-C18866

多克隆

domestic rabbit

生物素

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 免疫组化-石蜡切片

LS-C18866

Mammal

兔

胶原II抗体(生物素)LS-C18866

Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

多克隆

生物素

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

天然蛋白

Anti-Collagen Type II antibody was produced by repeated immunizations with Collagen Type II from adult human knee cartilage and bovine nasal cartilage.

亲和色谱

Store lyophilized at 4°C. Following resuspension aliquot and store at -20°C. Avoid freeze-thaw cycles and dilute immediately before use.

Reconstitute in 100 µL of deionized water.

IgG

胶原II

牛, 哺乳动物, 人类

酶联免疫吸附测定(1:10000 - 1:50000), 免疫组化, 免疫组化-P, 免疫沉淀(1:100), 免疫印迹(1:5000 - 1:10000)

Collagen II antibody LS-C18866 is a biotin-conjugated rabbit polyclonal antibody to Collagen II from mammal. It is reactive with human, bovine and mammal. Validated for ELISA, IHC, IP and WB.

Used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

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