抗体

胶原II抗体LS-C18868

LS-C18868

多克隆

domestic rabbit

未共轭

酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片

LS-C18868

Mammal

兔

胶原II抗体LS-C18868

Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

多克隆

未共轭

0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠, 0.01% 叠氮化钠

纯化蛋白

Collagen Type II from human knee cartilage and bovine nasal cartilage.

亲和色谱

Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.

IgG

胶原II

小鼠, 羊, 牛, 大鼠, 哺乳动物, 人类

酶联免疫吸附测定(1:5000 - 1:50000), 免疫组化, 免疫组化-P(1:50 - 1:400)

Collagen II antibody LS-C18868 is an unconjugated rabbit polyclonal antibody to Collagen II from mammal. It is reactive with human, mouse, rat and other species. Validated for ELISA and IHC. Cited in 1 publication.

Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and for Western Blot for highly sensitive qualitative analysis. This product was assayed by immunoblot and found to be reactive against Collagen II at a dilution of 1:5000 to 1:10000. This product was also assayed against 1.0 ug of Collagen II in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4000 to 1:8000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200.

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