产品简要
公司名称 :
LifeSpan Biosciences
产品类型 :
抗体
产品名称 :
AKT2抗体(aa450-475)LS-C19061
目录 :
LS-C19061
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 流式细胞仪
更多信息或购买 :
产品信息
抗体编号 :
19809
抗体名称 :
LS-C19061
目标物种 :
人类
宿主物种 :
兔
产品名称 :
AKT2抗体(aa450-475)LS-C19061
特异性 :
The antibody detects both unphosphorylated and phosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
克隆描述 :
多克隆
抗体修饰 :
未共轭
抗原修饰 :
aa450-475
样品状态 :
0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠, 0.01% 叠氮化钠
免疫原类型 :
合成肽
免疫原描述 :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near aa 450-475 (of 481) of human AKT2 conjugated to Keyhole Limpet Hemocyanin (KLH).
纯化描述 :
亲和纯化
推荐的储存条件 :
Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
抗体亚型 :
IgG
基因 :
AKT2
标准基因符号 :
AKT2
家族 :
蛋白激酶
亚家族 :
RAC/AKT
反应物种 :
人类
用途 :
ELISA (1:4000 - 1:16000), Flo, WB (1:1000)
ShortWebDescription :
AKT2 antibody LS-C19061 is an unconjugated rabbit polyclonal antibody to human AKT2 (aa450-475). Validated for ELISA, Flow and WB. Cited in 1 publication.
使用文本 :
This antibody was tested by ELISA and immunoblotting and was found to be reactive with both unphosphorylated and phosphorylated AKT2 in a lysate of HEK293 cells. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This product has been assayed by immunoblot against a HEK293 cell lysate and is reactive at a 1:1000 dilution showing a band at approximately 60 kD. A working dilution of 1:4000 to 1:16000 is suggested for this product in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature against 0.1 ug of the immunizing peptide. Researchers should determine optimal titers for other applications.
同义词 :
AKT2, PKB beta, PKBBETA, PRKBB, Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, Rac protein kinase beta, RAC-BETA, HIHGHH, PKBB
SalesRegion :
Worldwide
更多信息或购买 :
公司信息
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com1-206-464-1554
公司总部: 美国
LifeSpan成立于1995年，已经成为分子病理学领域的领导品牌，专注于人和其他物种的正常和疾病组织中蛋白的定位情况。我们提供超过74000种抗体，定制的免疫组化研究服务，超过500种蛋白研究用人免疫组化材料，及组化和病理学服务；提供2,000,000样本，包括冰冻及石蜡切片的正常和疾病组织，进行对外研究服务。对外服务项目范围广泛，由LifeSpan的病理学家提供包括课题设计、抗体搜寻及表征、组织搜集和验证、免疫标记、问题处理及结果处理等诸多环节。