抗体

NOTCH1抗体(N末端)LS-C19109

LS-C19109

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化

LS-C19109

人类

兔

NOTCH1抗体(N末端)LS-C19109

This antiserum is directed against human NOTCH 1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat NOTCH 1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.

多克隆

未共轭

N末端

0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠, 0.1% 叠氮化钠

合成肽

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).

抗血清

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

NOTCH1

NOTCH1

小鼠, 大鼠, 人类

酶联免疫吸附测定(1:20000 - 1:60000), 免疫组化(1:200), 免疫印迹(1:500 - 1:2000)

NOTCH1 antibody LS-C19109 is an unconjugated rabbit polyclonal antibody to NOTCH1 (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB. Cited in 3 publications.

This antibody is reactive with Notch 1 by western blotting. A strong response was also detected by ELISA against the immunizing peptide. This product was assayed by immunoblot against recombinant mouse Notch constructs in 293 cells. An 80 kD band corresponding to Notch 1 was observed at a 1:500 dilution. This product was also assayed against the peptide immunogen in a standard capture ELISA using Peroxidase conjugated anti-Rabbit IgG (H&L) [Goat] (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature.

NOTCH1, Notch 1, TAN1

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