Standard immunostaining protocol (red channel). The cells were also stained in green with BH7 a monoclonal antibody to Ubiquitin C-terminal Hydrolase 1 (UCHL1), an abundant cytoplasmic protein of neurons which is concentrated in the perikarya. Since the perikarya contain both UCHL1 and Tau, the red and green signals superimpose, giving a yellow color, while the processes, which contain relatively much more tau, appear red. Blue stain is DAPI and reveals cell nuclei of some non neuronal cells in these cultures.