抗体

AKT1抗体(内部, 克隆5E5.F5.D7)LS-C209208

LS-C209208

单克隆

小鼠

未共轭

5E5.F5.D7

免疫印迹, 酶联免疫吸附测定, 免疫组化, 流式细胞仪

LS-C209208

人类

小鼠

AKT1抗体(内部, 克隆5E5.F5.D7)LS-C209208

The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.

单克隆

5E5.F5.D7

未共轭

内部

0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠, 0.01% 叠氮化钠

合成肽

Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein.

蛋白质A亲和色谱

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

IgG2b, k

AKT1

AKT1

蛋白激酶

RAC/AKT

人类

ELISA (1:2000 - 1:10000), Flo, IHC (20 µg/ml), WB (1:500 - 1:2000)

AKT1 antibody LS-C209208 is an unconjugated mouse monoclonal antibody to human AKT1 (Internal). Validated for ELISA, Flow, IHC and WB.

Anti-AKT1 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kD in size corresponding to AKT1 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA

Worldwide