抗体

AKT2抗体(内部, 克隆11F6.B2)LS-C209268

LS-C209268

单克隆

大鼠

未共轭

11F6.B2

免疫印迹, 酶联免疫吸附测定, 免疫组化, 流式细胞仪

LS-C209268

人类

大鼠

AKT2抗体(内部, 克隆11F6.B2)LS-C209268

The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 was purified from concentrated tissue culture supernate by Protein A chromatography. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.

单克隆

11F6.B2

未共轭

内部

0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠

合成肽

Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.

蛋白质A亲和色谱

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

IgG2a, k

AKT2

AKT2

蛋白激酶

RAC/AKT

人类

ELISA (1:2000 - 1:10000), Flo, IHC (20 µg/ml), WB (1:500 - 1:2000)

AKT2 antibody LS-C209268 is an unconjugated rat monoclonal antibody to human AKT2 (Internal). Validated for ELISA, Flow, IHC and WB.

Anti-AKT2 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kD in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

AKT2, PKB beta, PKBBETA, PRKBB, Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, Rac protein kinase beta, RAC-BETA, HIHGHH, PKBB

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