免疫印迹, 免疫组化, 免疫细胞化学
Recognizes P2X2 Receptor. Species cross-reactivity: Rat and human.
PBS, 0.05% 叠氮化钠
Synthetic peptide corresponding to aa460-472 (DSTSTDPKGLAQL) from the C-Terminus of rat P2X2. Percent identity by BLAST analysis: Mouse, Rat (100%); Hamster, Guinea pig (92%); Human, Gorilla, Marmoset, Bat, Rabbit (85%).
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
免疫细胞化学(1:500), IF, 免疫组化(1:500), 免疫印迹(1:500)
P2X2 antibody LS-C25956 is an unconjugated guinea pig polyclonal antibody to P2X2 (P2RX2) (aa460-472) from rat. It is reactive with mouse and rat. Validated for ICC, IF, IHC and WB.
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Western Blot: 1:500. Cell membrane extracts were examined by electrophoresis (8% acrylamide) with SDS under reducing conditions and transferred to a nylon membrane. Membranes were blocked for 1 hour at 2-8?C with 0.1% Tween 20 and 2.5% milk powder (w/v) in PBS. Membranes were incubated and diluted 1:500 with same buffer overnight at 2-8?C. Membranes were rinsed and incubated with HRP conjugated secondary antibody for 1 hour at room temperature. Following rinsing the membranes were processed using enhanced chemiluminescence. Immunohistochemistry: 1:500. Male Sprague-Dawley rats (b. wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 ml of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8?C for 18-24 hours with antibody (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope. Immunocytochemistry: 1:500. 2X2 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as above.
P2RX2, ATP受体, p2X2, p2X受体, 亚单位2, 嘌呤受体, p2RX2, p2X受体2, p2X嘌呤受体2, p2X2嘌呤受体
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121