抗体

磷酸酪氨酸抗体(克隆PY20)LS-C57995

LS-C57995

单克隆

小鼠

未共轭

PY20

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

LS-C57995

Synthetic

小鼠

磷酸酪氨酸抗体(克隆PY20)LS-C57995

Recognizes phosphotyrosine, enabling the detection, characterization and isolation of proteins containing phosphorylated tyrosine residues. The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyze the transfer of ATP to a tyrosine residue on specific cell protein targets. The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine. The affinity of PY20 for phosphotyrosine is 10-6 to 10-7 M.

单克隆

PY20

未共轭

BBS, 0.09% 叠氮化钠

重组蛋白

亲和纯化

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

IgG2b

磷酸酪氨酸

Synthetic

酶联免疫吸附测定, IF, 免疫印迹(2微克/毫升)

Phosphotyrosine antibody LS-C57995 is an unconjugated mouse monoclonal antibody to synthetic Phosphotyrosine. Validated for ELISA, IF and WB.

Western Blot: For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1 mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Worldwide