抗体

肿瘤坏死因子甲抗体LS-C59380

LS-C59380

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学

LS-C59380

人类

兔

肿瘤坏死因子甲抗体LS-C59380

The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNFb (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.

多克隆

未共轭

抗血清

重组蛋白

The whole rabbit serum was prepared by repeated immunizations with recombinant human TNFa produced in E.coli.

抗血清

Short term: -20°C; Long term: -20°C.

肿瘤坏死因子甲

肿瘤坏死因子

肿瘤坏死因子

人类, 灵长类动物

酶联免疫吸附测定(1:1000 - 1:5000), IF(1:100), 免疫组化(1:100 - 1:500), 免疫印迹(1:500 - 1:2000)

TNF Alpha antibody LS-C59380 is an unconjugated rabbit polyclonal antibody to TNF Alpha from human. It is reactive with human and primate. Validated for ELISA, IF, IHC and WB.

This antiserum against Human TNFa has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting. It recognizes the 17000 MW TNFa. Reactivity in other immunoassays is unknown.

TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Worldwide