产品简要
公司名称 :
LifeSpan Biosciences
产品类型 :
抗体
产品名称 :
CDKN1A/WAF1/p21抗体(克隆WA-1)LS-C59514
目录 :
LS-C59514
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
WA-1
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 5
| 参考文献 |
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Joshi U, Chen Y, Kalemkerian G, Adil M, Kraut M, Sarkar F. Inhibition of tumor cell growth by p21WAF1 adenoviral gene transfer in lung cancer. Cancer Gene Ther. 1998;5:183-91 pubmed
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Kagawa S, Fujiwara T, Hizuta A, Yasuda T, Zhang W, Roth J, et al. p53 expression overcomes p21WAF1/CIP1-mediated G1 arrest and induces apoptosis in human cancer cells. Oncogene. 1997;15:1903-9 pubmed
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Yeh C, Shatkin A. A cis-acting element in Rous sarcoma virus long terminal repeat required for promoter repression by HeLa nuclear protein p21. J Biol Chem. 1995;270:15815-20 pubmed
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Waldman T, Kinzler K, Vogelstein B. p21 is necessary for the p53-mediated G1 arrest in human cancer cells. Cancer Res. 1995;55:5187-90 pubmed
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Yang Z, Perkins N, Ohno T, Nabel E, Nabel G. The p21 cyclin-dependent kinase inhibitor suppresses tumorigenicity in vivo. Nat Med. 1995;1:1052-6 pubmed
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产品信息
抗体编号 :
59984
抗体名称 :
LS-C59514
目标物种 :
人类
宿主物种 :
小鼠
产品名称 :
CDKN1A/WAF1/p21抗体(克隆WA-1)LS-C59514
克隆描述 :
单克隆
克隆名称 :
WA-1
抗体修饰 :
未共轭
样品状态 :
0.02 M钾磷酸盐, pH值7.2, 0.5 M氯化钠, 0.01% 叠氮化钠
免疫原类型 :
重组蛋白
免疫原描述 :
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.
纯化描述 :
蛋白质A纯化
推荐的储存条件 :
Short term: -20°C; Long term: -20°C.
抗体亚型 :
IgG1, k
基因 :
CDKN1A/WAF1/p21
标准基因符号 :
CDKN1A
反应物种 :
人类
用途 :
ELISA (1:5000 - 1:20000), IF (1:250), IHC, IHC-Fr, IHC-P, IP (1:100), WB (1:2000 - 1:10000)
ShortWebDescription :
P21 antibody LS-C59514 is an unconjugated mouse monoclonal antibody to human p21 (CDKN1A / WAF1). Validated for ELISA, IF, IHC, IP and WB. Cited in 5 publications.
使用文本 :
This antibody is suitable for immunohistochemistry, ELISA and immunoblotting. The antibody detects a 21 kD band corresponding to the p21 protein. MCF7 cells or UV treated fibroblasts are suggested as a positive control for immunoblotting and immunohistochemistry. Methanol/acetone fixed tissue is reactive for immunohistochemistry. The antibody also reacts with breast carcinoma on frozen sections (methanol/acetone fixation) and on paraffin sections (formalin fixation and microwave treatment prior to staining). In ELISA this monoclonal antibody is useful either as a capture antibody (attached to the solid phase) or for detection of p21 protein. This antibody immunoprecipitates monomeric p21 WAF1 and not p21 complexes with other proteins. High level expression of p21 WAF1 requires induction of wild-type p53 expression (e. g. via exposure to DNA damaging agents).
同义词 :
CDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21
SalesRegion :
Worldwide
更多信息或购买 :
公司信息
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com1-206-464-1554
公司总部: 美国
LifeSpan成立于1995年，已经成为分子病理学领域的领导品牌，专注于人和其他物种的正常和疾病组织中蛋白的定位情况。我们提供超过74000种抗体，定制的免疫组化研究服务，超过500种蛋白研究用人免疫组化材料，及组化和病理学服务；提供2,000,000样本，包括冰冻及石蜡切片的正常和疾病组织，进行对外研究服务。对外服务项目范围广泛，由LifeSpan的病理学家提供包括课题设计、抗体搜寻及表征、组织搜集和验证、免疫标记、问题处理及结果处理等诸多环节。