抗体

POU5F1/OCT4抗体(aa282-297)LS-C9379

LS-C9379

多克隆

domestic rabbit

未共轭

免疫印迹, 免疫组化, 免疫细胞化学

LS-C9379

人类

兔

POU5F1/OCT4抗体(aa282-297)LS-C9379

Recognizes Oct4 (POU5F1). MW: 38,.6kD. Species cross-reactivity: Human and rodent.

多克隆

未共轭

aa282-297

PBS, pH 7.4, No preservatives added

合成肽

Synthetic peptide (aa 282-297) of Oct4.

Immunoaffinity purified

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

IgG

POU5F1/OCT4

POU5F1

啮齿类, 人类

免疫细胞化学(1:200 - 1:1000), 免疫组化(1:200 - 1:1000), 免疫印迹(1:200 - 1:1000)

OCT4 antibody LS-C9379 is an unconjugated rabbit polyclonal antibody to OCT4 (POU5F1) (aa282-297) from human. It is reactive with human and rodent. Validated for ICC, IHC and WB.

Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:200-1:1000 using ECL. Immunohistochemistry: 1:200-1:1000. Immunocytochemistry: 1:200-1:1000.Immunohistochemistry protocol:1. Dissect tissues and freeze on dry ice.2. Cut on a cryostat 10um slices.3. Dry slides at RT for 30-90 min4. Fix with cold Acetone: Methanol (50:50) 2 min. Either process further or store at -20ºC. Alternative is fixation with 4% PFA and treatment with 0.05% trypsin solution (incubation 10 min. at 37ºC, followed by 3 washes with PBS)5. Let air dry.6. PBS 5 min7. Incubate in 50mM ammonium chloride 30 min.8. PBS 5 min9. Blocking serum 30-45 min10. Primary antibody (LS-C9379) 90 min11. Wash 3X for 5 min12. Secondary fluorescent conjugated antibody 30 min13. Wash 3X for 5 min.14. Mount.

POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3, OCT3/4

Worldwide