产品简要
公司名称 :
MABTECH
产品类型 :
ELISA/assay
产品名称 :
FluoroSpot Plus: Human IFN-gamma/IL-2
目录 :
FSP-0102-2
规格 :
2孔板
价格 :
890美元
更多信息或购买 :
MABTECH 产品网页
文章摘录数: 19
参考文献
Tarke A, Sidney J, Methot N, Yu E, Zhang Y, Dan J, et al. Impact of SARS-CoV-2 variants on the total CD4+ and CD8+ T cell reactivity in infected or vaccinated individuals. Cell Rep Med. 2021;2:100355 pubmed 出版商
Singhania A, Pham J, Dhanwani R, Frazier A, Rezende Dutra J, Marder K, et al. The TCR repertoire of α-synuclein-specific T cells in Parkinson's disease is surprisingly diverse. Sci Rep. 2021;11:302 pubmed 出版商
Mateus J, Grifoni A, Tarke A, Sidney J, Ramirez S, Dan J, et al. Selective and cross-reactive SARS-CoV-2 T cell epitopes in unexposed humans. Science. 2020;370:89-94 pubmed 出版商
Fostier K, Caers J, Meuleman N, Broos K, Corthals J, Thielemans K, et al. Impact of lenalidomide maintenance on the immune environment of multiple myeloma patients with low tumor burden after autologous stem cell transplantation. Oncotarget. 2018;9:20476-20489 pubmed 出版商
Carrasco Pro S, Lindestam Arlehamn C, Dhanda S, Carpenter C, Lindvall M, Faruqi A, et al. Microbiota epitope similarity either dampens or enhances the immunogenicity of disease-associated antigenic epitopes. PLoS ONE. 2018;13:e0196551 pubmed 出版商
Grist J, Jarvis L, Georgieva Z, Thompson S, Kaur Sandhu H, Burling K, et al. Extracellular Lactate: A Novel Measure of T Cell Proliferation. J Immunol. 2018;200:1220-1226 pubmed 出版商
Lund G, Brand S, Ramos T, Jimeno L, Boissy P, Vega F, et al. Strong and frequent T-cell responses to the minor allergen Phl p 12 in Spanish patients IgE-sensitized to Profilins. Allergy. 2018;73:1013-1021 pubmed 出版商
Das A, Rouault Pierre K, Kamdar S, Gomez Tourino I, Wood K, Donaldson I, et al. Adaptive from Innate: Human IFN-γ+CD4+ T Cells Can Arise Directly from CXCL8-Producing Recent Thymic Emigrants in Babies and Adults. J Immunol. 2017;199:1696-1705 pubmed 出版商
Jackson S, Sedikides G, Okecha G, Poole E, Sinclair J, Wills M. Latent Cytomegalovirus (CMV) Infection Does Not Detrimentally Alter T Cell Responses in the Healthy Old, But Increased Latent CMV Carriage Is Related to Expanded CMV-Specific T Cells. Front Immunol. 2017;8:733 pubmed 出版商
Danielzik T, Koldehoff M, Buttkereit U, Beelen D, Horn P, Lindemann M. Sensitive detection of rare antigen-specific T cells directed against Wilms' tumor 1 by FluoroSpot assay. Leuk Lymphoma. 2018;59:490-492 pubmed 出版商
Uebele J, Stein C, Nguyen M, Schneider A, Kleinert F, Ticha O, et al. Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus. PLoS Pathog. 2017;13:e1006387 pubmed 出版商
Jackson S, Sedikides G, Mason G, Okecha G, Wills M. Human Cytomegalovirus (HCMV)-Specific CD4+ T Cells Are Polyfunctional and Can Respond to HCMV-Infected Dendritic Cells In Vitro. J Virol. 2017;91: pubmed 出版商
Clement M, Marsden M, Stacey M, Abdul Karim J, Gimeno Brias S, Costa Bento D, et al. Cytomegalovirus-Specific IL-10-Producing CD4+ T Cells Are Governed by Type-I IFN-Induced IL-27 and Promote Virus Persistence. PLoS Pathog. 2016;12:e1006050 pubmed 出版商
Körber N, Behrends U, Hapfelmeier A, Protzer U, Bauer T. Validation of an IFN?/IL2 FluoroSpot assay for clinical trial monitoring. J Transl Med. 2016;14:175 pubmed 出版商
Brunskole Hummel I, Zitzmann A, Erl M, Wenzel J, Jilg W. Characteristics of immune memory 10-15 years after primary hepatitis B vaccination. Vaccine. 2016;34:636-642 pubmed 出版商
Brunskole Hummel I, Huber B, Wenzel J, Jilg W. Markers of Protection in Children and Adolescents Six to Fourteen Years After Primary Hepatitis B Vaccination in Real Life: A Pilot Study. Pediatr Infect Dis J. 2016;35:286-91 pubmed 出版商
Dillenbeck T, Gelius E, Fohlstedt J, Ahlborg N. Triple Cytokine FluoroSpot Analysis of Human Antigen-Specific IFN-γ, IL-17A and IL-22 Responses. Cells. 2014;3:1116-30 pubmed 出版商
Janetzki S, Rueger M, Dillenbeck T. Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays. Cells. 2014;3:1102-15 pubmed 出版商
Ohrmalm L, Smedman C, Wong M, Broliden K, Tolfvenstam T, Norbeck O. Decreased functional T lymphocyte-mediated cytokine responses in patients with chemotherapy-induced neutropenia. J Intern Med. 2013;274:363-70 pubmed 出版商
产品信息
产品代码 :
FSP-0102-2
产品名称 :
FluoroSpot Plus: Human IFN-gamma/IL-2
Categories :
FluoroSpot Kits
UNSPSC类别 :
Protein chemifluorescent detection reagents or kits or substrates
UNSPSC编码 :
41105331
规格 :
2孔板
规格数目 :
2
尺寸单位 :
plates
2023 USD List :
890美元
保存期限 :
12 months
Last updated :
2013-07-01
格式 :
pre-coated plates
细胞类型 :
T细胞
应用 :
FluoroSpot
Reactivities :
人类
Analytes :
IFNG, 白细胞介素
Recommended Products :
FSP-0102-10
Contents :
BAM-conjugated detection mAb (7-B6-1),Biotinylated detection mAb (MT8G10) ,Anti-BAM-490,SA-550,Anti-CD3 mAb,Anti-CD28 mAb (CD28-A),FluoroSpot enhancer,Pre-coated FluoroSpot plates (mAbs 1-D1K and MT2A91/2C95)
Product Header :
FluoroSpot kit with pre-coated plates for enumeration of cells secreting IFN-γ and IL-2
描述1 :
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/IL-2 FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting either one or both analytes and includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.
描述2 :
Pre-coated plates enable a more rapid assay with minimized variability.
参考文献 :
Uebele, J., et al. (2017). Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus. PLoS Pathog. (http://www.ncbi.nlm.nih.gov/pubmed/28542586) Fostier, K., et al. (2018). Impact of lenalidomide maintenance on the immune environment of multiple myeloma patients with low tumor burden after autologous stem cell transplantation. Oncotarget. (http://www.ncbi.nlm.nih.gov/pubmed/29755666) Dillenbeck, T., et al. (2014). Triple Cytokine FluoroSpot Analysis of Human Antigen-Specific IFN-gamma, IL-17A and IL-22 Responses. Cells. (http://www.ncbi.nlm.nih.gov/pubmed/25437441) Mateus, J., et al. (2020). Selective and cross-reactive SARS-CoV-2 T cell epitopes in unexposed humans. Science. (http://www.ncbi.nlm.nih.gov/pubmed/32753554) Tarke, A., et al. (2021). Impact of SARS-CoV-2 variants on the total CD4(+) and CD8(+) T cell reactivity in infected or vaccinated individuals. Cell Rep Med. (http://www.ncbi.nlm.nih.gov/pubmed/34230917)
更多信息或购买 :
MABTECH 产品网页
公司信息
MABTECH
Box 1233
SE-131 28 Nacka Strand
mabtech@mabtech.com
http://www.mabtech.com
46-8-716-27-00
公司总部: 瑞典