产品简要
公司名称 :
MABTECH
产品类型 :
ELISA/assay
产品名称 :
FluoroSpot Plus: Human IFN-gamma/IL-2/TNF-alpha
目录 :
FSP-010209-10
规格 :
10孔板
价格 :
4955美元
更多信息或购买 :
MABTECH 产品网页
文章摘录数: 18
参考文献
Foord E, Arruda L, Gaballa A, Klynning C, Uhlin M. Characterization of ascites- and tumor-infiltrating γδ T cells reveals distinct repertoires and a beneficial role in ovarian cancer. Sci Transl Med. 2021;13: pubmed 出版商
Singhania A, Pham J, Dhanwani R, Frazier A, Rezende Dutra J, Marder K, et al. The TCR repertoire of α-synuclein-specific T cells in Parkinson's disease is surprisingly diverse. Sci Rep. 2021;11:302 pubmed 出版商
Gibani M, Jones E, Barton A, Jin C, Meek J, Camara S, et al. Investigation of the role of typhoid toxin in acute typhoid fever in a human challenge model. Nat Med. 2019;25:1082-1088 pubmed 出版商
Bronge M, Ruhrmann S, Carvalho Queiroz C, Nilsson O, Kaiser A, Holmgren E, et al. Myelin oligodendrocyte glycoprotein revisited-sensitive detection of MOG-specific T-cells in multiple sclerosis. J Autoimmun. 2019;: pubmed 出版商
Birrueta G, Frazier A, Pomes A, Glesner J, Filep S, Schal C, et al. Variability in German Cockroach Extract Composition Greatly Impacts T Cell Potency in Cockroach-Allergic Donors. Front Immunol. 2019;10:313 pubmed 出版商
Fostier K, Caers J, Meuleman N, Broos K, Corthals J, Thielemans K, et al. Impact of lenalidomide maintenance on the immune environment of multiple myeloma patients with low tumor burden after autologous stem cell transplantation. Oncotarget. 2018;9:20476-20489 pubmed 出版商
Lund G, Brand S, Ramos T, Jimeno L, Boissy P, Vega F, et al. Strong and frequent T-cell responses to the minor allergen Phl p 12 in Spanish patients IgE-sensitized to Profilins. Allergy. 2018;73:1013-1021 pubmed 出版商
Scurr M, Pembroke T, Bloom A, Roberts D, Thomson A, Smart K, et al. Low-Dose Cyclophosphamide Induces Antitumor T-Cell Responses, which Associate with Survival in Metastatic Colorectal Cancer. Clin Cancer Res. 2017;23:6771-6780 pubmed 出版商
Das A, Rouault Pierre K, Kamdar S, Gomez Tourino I, Wood K, Donaldson I, et al. Adaptive from Innate: Human IFN-γ+CD4+ T Cells Can Arise Directly from CXCL8-Producing Recent Thymic Emigrants in Babies and Adults. J Immunol. 2017;199:1696-1705 pubmed 出版商
Clement M, Marsden M, Stacey M, Abdul Karim J, Gimeno Brias S, Costa Bento D, et al. Cytomegalovirus-Specific IL-10-Producing CD4+ T Cells Are Governed by Type-I IFN-Induced IL-27 and Promote Virus Persistence. PLoS Pathog. 2016;12:e1006050 pubmed 出版商
Brunskole Hummel I, Zitzmann A, Erl M, Wenzel J, Jilg W. Characteristics of immune memory 10-15 years after primary hepatitis B vaccination. Vaccine. 2016;34:636-642 pubmed 出版商
Dillenbeck T, Gelius E, Fohlstedt J, Ahlborg N. Triple Cytokine FluoroSpot Analysis of Human Antigen-Specific IFN-γ, IL-17A and IL-22 Responses. Cells. 2014;3:1116-30 pubmed 出版商
Janetzki S, Rueger M, Dillenbeck T. Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays. Cells. 2014;3:1102-15 pubmed 出版商
Han W, van Twillert I, Poelen M, Helm K, van de Kassteele J, Verheij T, et al. Loss of multi-epitope specificity in memory CD4(+) T cell responses to B. pertussis with age. PLoS ONE. 2013;8:e83583 pubmed 出版商
Braesch Andersen S, Paulie S, Smedman C, Mia S, Kumagai Braesch M. ApoE production in human monocytes and its regulation by inflammatory cytokines. PLoS ONE. 2013;8:e79908 pubmed 出版商
Ohrmalm L, Smedman C, Wong M, Broliden K, Tolfvenstam T, Norbeck O. Decreased functional T lymphocyte-mediated cytokine responses in patients with chemotherapy-induced neutropenia. J Intern Med. 2013;274:363-70 pubmed 出版商
Smedman C, Ernemar T, Gudmundsdotter L, Gille Johnson P, Somell A, Nihlmark K, et al. FluoroSpot Analysis of TLR-Activated Monocytes Reveals Several Distinct Cytokine-Secreting Subpopulations. Scand J Immunol. 2012;75:249-58 pubmed 出版商
Lee F, Walsh E, Falsey A, Lumb M, Okam N, Liu N, et al. Human infant respiratory syncytial virus (RSV)-specific type 1 and 2 cytokine responses ex vivo during primary RSV infection. J Infect Dis. 2007;195:1779-88 pubmed
产品信息
产品代码 :
FSP-010209-10
产品名称 :
FluoroSpot Plus: Human IFN-gamma/IL-2/TNF-alpha
Categories :
FluoroSpot Kits
UNSPSC类别 :
Protein chemifluorescent detection reagents or kits or substrates
UNSPSC编码 :
41105331
规格 :
10孔板
规格数目 :
10
尺寸单位 :
plates
2023 USD List :
4955美元
保存期限 :
12 months
Last updated :
2016-04-11
格式 :
pre-coated plates
应用 :
FluoroSpot
Reactivities :
人类
Analytes :
IFNG, 白细胞介素, TNFA
Recommended Products :
FSP-0109-2
Contents :
BAM-conjugated detection mAb (7-B6-1),Biotinylated detection mAb (MT8G10) ,WASP-conjugated detection mAb (MT20D9),Anti-BAM-490,SA-550,Anti-WASP-640,FluoroSpot enhancer,Anti-CD3 mAb,Anti-CD28 mAb (CD28-A),Pre-coated FluoroSpot plates (mAbs 1-D1K, MT2A91/2C95 and MT25C5)
Product Header :
FluoroSpot kit with pre-coated plates for enumeration of cells secreting IFN-γ, IL-2 and TNF-α
描述1 :
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/IL-2/ TNF-α FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting one, two or three analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.
描述2 :
Pre-coated plates enable a more rapid assay with minimized variability.
参考文献 :
Smedman, C., et al. (2011). FluoroSpot analysis of TLR-activated monocytes reveals several distinct cytokine secreting subpopulations. Scandinavian journal of immunology. (http://www.ncbi.nlm.nih.gov/pubmed/21955279) Bronge, M., et al. (2019). Myelin oligodendrocyte glycoprotein revisited-sensitive detection of MOG-specific T-cells in multiple sclerosis. Journal of autoimmunity. (http://www.ncbi.nlm.nih.gov/pubmed/31054941) Lee, F. E., et al. (2007). Human infant respiratory syncytial virus (RSV)-specific type 1 and 2 cytokine responses ex vivo during primary RSV infection. The Journal of infectious diseases. (http://www.ncbi.nlm.nih.gov/pubmed/17492594) Foord, E., et al. (2021). Characterization of ascites- and tumor-infiltrating gammadelta T cells reveals distinct repertoires and a beneficial role in ovarian cancer. Sci Transl Med. (http://www.ncbi.nlm.nih.gov/pubmed/33472952) Singhania, A., et al. (2021). The TCR repertoire of alpha-synuclein-specific T cells in Parkinson's disease is surprisingly diverse. Sci Rep. (http://www.ncbi.nlm.nih.gov/pubmed/33432042)
更多信息或购买 :
MABTECH 产品网页
公司信息
MABTECH
Box 1233
SE-131 28 Nacka Strand
mabtech@mabtech.com
http://www.mabtech.com
46-8-716-27-00
公司总部: 瑞典