产品简要
公司名称 :
MABTECH
产品类型 :
ELISA/assay
产品名称 :
FluoroSpot Plus: Human IFN-gamma/IL-10
目录 :
FSP-0107-2
规格 :
2孔板
价格 :
890美元
更多信息或购买 :
文章摘录数: 22
参考文献
Bronge M, H xf6 gelin K, Thomas O, Ruhrmann S, Carvalho Queiroz C, Nilsson O, et al. Identification of four novel T cell autoantigens and personal autoreactive profiles in multiple sclerosis. Sci Adv. 2022;8:eabn1823 pubmed 出版商
Sandberg J, Varnaitė R, Christ W, Chen P, Muvva J, Maleki K, et al. SARS-CoV-2-specific humoral and cellular immunity persists through 9 months irrespective of COVID-19 severity at hospitalisation. Clin Transl Immunology. 2021;10:e1306 pubmed 出版商
Thomson A, Bento D, Scurr M, Smart K, Somerville M, Keita A, et al. Prognostic significance of interleukin-17A-producing colorectal tumour antigen-specific T cells. Br J Cancer. 2021;124:1552-1555 pubmed 出版商
Leuchte K, Staib E, Thelen M, Gödel P, Lechner A, Zentis P, et al. Microwave ablation enhances tumor-specific immune response in patients with hepatocellular carcinoma. Cancer Immunol Immunother. 2021;70:893-907 pubmed 出版商
Gibani M, Jones E, Barton A, Jin C, Meek J, Camara S, et al. Investigation of the role of typhoid toxin in acute typhoid fever in a human challenge model. Nat Med. 2019;25:1082-1088 pubmed 出版商
Bronge M, Ruhrmann S, Carvalho Queiroz C, Nilsson O, Kaiser A, Holmgren E, et al. Myelin oligodendrocyte glycoprotein revisited-sensitive detection of MOG-specific T-cells in multiple sclerosis. J Autoimmun. 2019;: pubmed 出版商
Birrueta G, Frazier A, Pomes A, Glesner J, Filep S, Schal C, et al. Variability in German Cockroach Extract Composition Greatly Impacts T Cell Potency in Cockroach-Allergic Donors. Front Immunol. 2019;10:313 pubmed 出版商
Levin M, Kroehl M, Johnson M, Hammes A, Reinhold D, Lang N, et al. Th1 memory differentiates recombinant from live herpes zoster vaccines. J Clin Invest. 2018;128:4429-4440 pubmed 出版商
Fostier K, Caers J, Meuleman N, Broos K, Corthals J, Thielemans K, et al. Impact of lenalidomide maintenance on the immune environment of multiple myeloma patients with low tumor burden after autologous stem cell transplantation. Oncotarget. 2018;9:20476-20489 pubmed 出版商
Scurr M, Pembroke T, Bloom A, Roberts D, Thomson A, Smart K, et al. Low-Dose Cyclophosphamide Induces Antitumor T-Cell Responses, which Associate with Survival in Metastatic Colorectal Cancer. Clin Cancer Res. 2017;23:6771-6780 pubmed 出版商
Danielzik T, Koldehoff M, Buttkereit U, Beelen D, Horn P, Lindemann M. Sensitive detection of rare antigen-specific T cells directed against Wilms' tumor 1 by FluoroSpot assay. Leuk Lymphoma. 2018;59:490-492 pubmed 出版商
Uebele J, Stein C, Nguyen M, Schneider A, Kleinert F, Ticha O, et al. Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus. PLoS Pathog. 2017;13:e1006387 pubmed 出版商
Jackson S, Sedikides G, Mason G, Okecha G, Wills M. Human Cytomegalovirus (HCMV)-Specific CD4+ T Cells Are Polyfunctional and Can Respond to HCMV-Infected Dendritic Cells In Vitro. J Virol. 2017;91: pubmed 出版商
Clement M, Marsden M, Stacey M, Abdul Karim J, Gimeno Brias S, Costa Bento D, et al. Cytomegalovirus-Specific IL-10-Producing CD4+ T Cells Are Governed by Type-I IFN-Induced IL-27 and Promote Virus Persistence. PLoS Pathog. 2016;12:e1006050 pubmed 出版商
Kaiser Y, Lepzien R, Kullberg S, Eklund A, Smed Sörensen A, Grunewald J. Expanded lung T-bet+RORγT+ CD4+ T-cells in sarcoidosis patients with a favourable disease phenotype. Eur Respir J. 2016;48:484-94 pubmed 出版商
Brunskole Hummel I, Zitzmann A, Erl M, Wenzel J, Jilg W. Characteristics of immune memory 10-15 years after primary hepatitis B vaccination. Vaccine. 2016;34:636-642 pubmed 出版商
Janetzki S, Rueger M, Dillenbeck T. Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays. Cells. 2014;3:1102-15 pubmed 出版商
Han W, van Twillert I, Poelen M, Helm K, van de Kassteele J, Verheij T, et al. Loss of multi-epitope specificity in memory CD4(+) T cell responses to B. pertussis with age. PLoS ONE. 2013;8:e83583 pubmed 出版商
Chauvat A, Benhamouda N, Gey A, Lemoine F, Paulie S, Carrat F, et al. Clinical validation of IFN?/IL-10 and IFN?/IL-2 FluoroSpot assays for the detection of Tr1 T cells and influenza vaccine monitoring in humans. Hum Vaccin Immunother. 2014;10:104-13 pubmed 出版商
Sridhar S, Begom S, Bermingham A, Hoschler K, Adamson W, Carman W, et al. Cellular immune correlates of protection against symptomatic pandemic influenza. Nat Med. 2013;19:1305-12 pubmed 出版商
Ohrmalm L, Smedman C, Wong M, Broliden K, Tolfvenstam T, Norbeck O. Decreased functional T lymphocyte-mediated cytokine responses in patients with chemotherapy-induced neutropenia. J Intern Med. 2013;274:363-70 pubmed 出版商
Lee F, Walsh E, Falsey A, Lumb M, Okam N, Liu N, et al. Human infant respiratory syncytial virus (RSV)-specific type 1 and 2 cytokine responses ex vivo during primary RSV infection. J Infect Dis. 2007;195:1779-88 pubmed
产品信息
产品代码 :
FSP-0107-2
产品名称 :
FluoroSpot Plus: Human IFN-gamma/IL-10
Categories :
FluoroSpot Kits
UNSPSC类别 :
Protein chemifluorescent detection reagents or kits or substrates
UNSPSC编码 :
41105331
规格 :
2孔板
规格数目 :
2
尺寸单位 :
plates
2023 USD List :
890美元
保存期限 :
12 months
Last updated :
2014-10-23
格式 :
pre-coated plates
细胞类型 :
T细胞
应用 :
FluoroSpot
Reactivities :
人类
Analytes :
IFNG, 白细胞介素10
Recommended Products :
FSP-010703-2
Contents :
BAM-conjugated detection mAb (7-B6-1),Biotinylated detection mAb (12G8),Anti-BAM-490,SA-550,Anti-CD3 mAb,Anti-CD28 mAb (CD28-A),FluoroSpot enhancer,Pre-coated FluoroSpot plates (mAbs 1-D1K and 9D7)
Product Header :
FluoroSpot kit with pre-coated plates for enumeration of cells secreting IFN-γ and IL-10
描述1 :
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/IL-10 FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting either one or both analytes and includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.
描述2 :
Pre-coated plates enable a more rapid assay with minimized variability.
参考文献 :
Bronge, M., et al. (2022). Identification of four novel T cell autoantigens and personal autoreactive profiles in multiple sclerosis. Sci Adv. (http://www.ncbi.nlm.nih.gov/pubmed/35476434) Uebele, J., et al. (2017). Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus. PLoS Pathog. (http://www.ncbi.nlm.nih.gov/pubmed/28542586) Gibani, M. M., et al. (2019). Investigation of the role of typhoid toxin in acute typhoid fever in a human challenge model. Nature medicine. (http://www.ncbi.nlm.nih.gov/pubmed/31270506) Sandberg, J. T., et al. (2021). SARS-CoV-2-specific humoral and cellular immunity persists through 9 months irrespective of COVID-19 severity at hospitalisation. Clin Transl Immunology. (http://www.ncbi.nlm.nih.gov/pubmed/34257967) Thomson, A., et al. (2021). Prognostic significance of interleukin-17A-producing colorectal tumour antigen-specific T cells. British journal of cancer. (http://www.ncbi.nlm.nih.gov/pubmed/33674735)
更多信息或购买 :
公司信息
MABTECH
Box 1233
SE-131 28 Nacka Strand
mabtech@mabtech.com
http://www.mabtech.com
46-8-716-27-00
公司总部: 瑞典