产品简要
公司名称 :
MABTECH
产品类型 :
ELISA/assay
产品名称 :
FluoroSpot Plus: Human IFN-gamma/IL-10/IL-17A
目录 :
FSP-010703-10
规格 :
10孔板
价格 :
4955美元
更多信息或购买 :
MABTECH 产品网页
文章摘录数: 23
参考文献
Tarke A, Sidney J, Methot N, Yu E, Zhang Y, Dan J, et al. Impact of SARS-CoV-2 variants on the total CD4+ and CD8+ T cell reactivity in infected or vaccinated individuals. Cell Rep Med. 2021;2:100355 pubmed 出版商
Thomson A, Bento D, Scurr M, Smart K, Somerville M, Keita A, et al. Prognostic significance of interleukin-17A-producing colorectal tumour antigen-specific T cells. Br J Cancer. 2021;124:1552-1555 pubmed 出版商
Singhania A, Pham J, Dhanwani R, Frazier A, Rezende Dutra J, Marder K, et al. The TCR repertoire of α-synuclein-specific T cells in Parkinson's disease is surprisingly diverse. Sci Rep. 2021;11:302 pubmed 出版商
Lindestam Arlehamn C, Dhanwani R, Pham J, Kuan R, Frazier A, Rezende Dutra J, et al. α-Synuclein-specific T cell reactivity is associated with preclinical and early Parkinson's disease. Nat Commun. 2020;11:1875 pubmed 出版商
Smith C, Moraka N, Ibrahim M, Moyo S, Mayondi G, Kammerer B, et al. Human Immunodeficiency Virus Exposure but Not Early Cytomegalovirus Infection Is Associated With Increased Hospitalization and Decreased Memory T-Cell Responses to Tetanus Vaccine. J Infect Dis. 2020;221:1167-1175 pubmed 出版商
Gibani M, Jones E, Barton A, Jin C, Meek J, Camara S, et al. Investigation of the role of typhoid toxin in acute typhoid fever in a human challenge model. Nat Med. 2019;25:1082-1088 pubmed 出版商
Bronge M, Ruhrmann S, Carvalho Queiroz C, Nilsson O, Kaiser A, Holmgren E, et al. Myelin oligodendrocyte glycoprotein revisited-sensitive detection of MOG-specific T-cells in multiple sclerosis. J Autoimmun. 2019;: pubmed 出版商
Birrueta G, Frazier A, Pomes A, Glesner J, Filep S, Schal C, et al. Variability in German Cockroach Extract Composition Greatly Impacts T Cell Potency in Cockroach-Allergic Donors. Front Immunol. 2019;10:313 pubmed 出版商
Jelcić I, Al Nimer F, Wang J, Lentsch V, Planas R, Jelcic I, et al. Memory B Cells Activate Brain-Homing, Autoreactive CD4+ T Cells in Multiple Sclerosis. Cell. 2018;175:85-100.e23 pubmed 出版商
Levin M, Kroehl M, Johnson M, Hammes A, Reinhold D, Lang N, et al. Th1 memory differentiates recombinant from live herpes zoster vaccines. J Clin Invest. 2018;128:4429-4440 pubmed 出版商
Carrasco Pro S, Lindestam Arlehamn C, Dhanda S, Carpenter C, Lindvall M, Faruqi A, et al. Microbiota epitope similarity either dampens or enhances the immunogenicity of disease-associated antigenic epitopes. PLoS ONE. 2018;13:e0196551 pubmed 出版商
Grist J, Jarvis L, Georgieva Z, Thompson S, Kaur Sandhu H, Burling K, et al. Extracellular Lactate: A Novel Measure of T Cell Proliferation. J Immunol. 2018;200:1220-1226 pubmed 出版商
Das A, Rouault Pierre K, Kamdar S, Gomez Tourino I, Wood K, Donaldson I, et al. Adaptive from Innate: Human IFN-γ+CD4+ T Cells Can Arise Directly from CXCL8-Producing Recent Thymic Emigrants in Babies and Adults. J Immunol. 2017;199:1696-1705 pubmed 出版商
Jackson S, Sedikides G, Okecha G, Poole E, Sinclair J, Wills M. Latent Cytomegalovirus (CMV) Infection Does Not Detrimentally Alter T Cell Responses in the Healthy Old, But Increased Latent CMV Carriage Is Related to Expanded CMV-Specific T Cells. Front Immunol. 2017;8:733 pubmed 出版商
Danielzik T, Koldehoff M, Buttkereit U, Beelen D, Horn P, Lindemann M. Sensitive detection of rare antigen-specific T cells directed against Wilms' tumor 1 by FluoroSpot assay. Leuk Lymphoma. 2018;59:490-492 pubmed 出版商
Uebele J, Stein C, Nguyen M, Schneider A, Kleinert F, Ticha O, et al. Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus. PLoS Pathog. 2017;13:e1006387 pubmed 出版商
Clement M, Marsden M, Stacey M, Abdul Karim J, Gimeno Brias S, Costa Bento D, et al. Cytomegalovirus-Specific IL-10-Producing CD4+ T Cells Are Governed by Type-I IFN-Induced IL-27 and Promote Virus Persistence. PLoS Pathog. 2016;12:e1006050 pubmed 出版商
Kaiser Y, Lepzien R, Kullberg S, Eklund A, Smed Sörensen A, Grunewald J. Expanded lung T-bet+RORγT+ CD4+ T-cells in sarcoidosis patients with a favourable disease phenotype. Eur Respir J. 2016;48:484-94 pubmed 出版商
Brunskole Hummel I, Zitzmann A, Erl M, Wenzel J, Jilg W. Characteristics of immune memory 10-15 years after primary hepatitis B vaccination. Vaccine. 2016;34:636-642 pubmed 出版商
Brunskole Hummel I, Huber B, Wenzel J, Jilg W. Markers of Protection in Children and Adolescents Six to Fourteen Years After Primary Hepatitis B Vaccination in Real Life: A Pilot Study. Pediatr Infect Dis J. 2016;35:286-91 pubmed 出版商
Dillenbeck T, Gelius E, Fohlstedt J, Ahlborg N. Triple Cytokine FluoroSpot Analysis of Human Antigen-Specific IFN-γ, IL-17A and IL-22 Responses. Cells. 2014;3:1116-30 pubmed 出版商
Janetzki S, Rueger M, Dillenbeck T. Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays. Cells. 2014;3:1102-15 pubmed 出版商
Sridhar S, Begom S, Bermingham A, Hoschler K, Adamson W, Carman W, et al. Cellular immune correlates of protection against symptomatic pandemic influenza. Nat Med. 2013;19:1305-12 pubmed 出版商
产品信息
产品代码 :
FSP-010703-10
产品名称 :
FluoroSpot Plus: Human IFN-gamma/IL-10/IL-17A
Categories :
FluoroSpot Kits
UNSPSC类别 :
Protein chemifluorescent detection reagents or kits or substrates
UNSPSC编码 :
41105331
规格 :
10孔板
规格数目 :
10
尺寸单位 :
plates
2023 USD List :
4955美元
保存期限 :
12 months
Last updated :
2014-11-06
格式 :
pre-coated plates
细胞类型 :
T细胞
应用 :
FluoroSpot
Reactivities :
人类
Analytes :
IFNG, 白细胞介素10, IL17A
Recommended Products :
FSP-18010703-10
Contents :
BAM-conjugated detection mAb (7-B6-1),Biotinylated detection mAb (12G8),WASP-conjugated detection mAb (MT504) ,Anti-BAM-490,SA-550,Anti-WASP-640,Anti-CD3 mAb,Anti-CD28 mAb (CD28-A),FluoroSpot enhancer,Pre-coated FluoroSpot plates (mAbs 1-D1K, 9D7 and MT44.6)
Product Header :
FluoroSpot kit with pre-coated plates for enumeration of cells secreting IFN-γ, IL-10 and IL-17A
描述1 :
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/IL-10/IL-17A FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting one, two or three analytes and includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.
描述2 :
Pre-coated plates enable a more rapid assay with minimized variability.
参考文献 :
Smith, C., et al. (2019). HIV Exposure but Not Early CMV Infection is Associated with Increased Hospitalization and Decreased Memory T-cell Responses to Tetanus Vaccine. The Journal of infectious diseases. (http://www.ncbi.nlm.nih.gov/pubmed/31711179) Uebele, J., et al. (2017). Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus. PLoS Pathog. (http://www.ncbi.nlm.nih.gov/pubmed/28542586) Lindestam Arlehamn, C. S., et al. (2020). alpha-Synuclein-specific T cell reactivity is associated with preclinical and early Parkinson's disease. Nat Commun. (http://www.ncbi.nlm.nih.gov/pubmed/32313102) Thomson, A., et al. (2021). Prognostic significance of interleukin-17A-producing colorectal tumour antigen-specific T cells. British journal of cancer. (http://www.ncbi.nlm.nih.gov/pubmed/33674735) Bronge, M., et al. (2019). Myelin oligodendrocyte glycoprotein revisited-sensitive detection of MOG-specific T-cells in multiple sclerosis. Journal of autoimmunity. (http://www.ncbi.nlm.nih.gov/pubmed/31054941)
更多信息或购买 :
MABTECH 产品网页
公司信息
MABTECH
Box 1233
SE-131 28 Nacka Strand
mabtech@mabtech.com
http://www.mabtech.com
46-8-716-27-00
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