产品简要
公司名称 :
MABTECH
产品类型 :
ELISA/assay
产品名称 :
FluoroSpot Plus: Human IFN-gamma/TNF-alpha
目录 :
FSP-0109-10
规格 :
10孔板
价格 :
3885美元
更多信息或购买 :
文章摘录数: 24
参考文献
Bronge M, H xf6 gelin K, Thomas O, Ruhrmann S, Carvalho Queiroz C, Nilsson O, et al. Identification of four novel T cell autoantigens and personal autoreactive profiles in multiple sclerosis. Sci Adv. 2022;8:eabn1823 pubmed 出版商
Thomson A, Bento D, Scurr M, Smart K, Somerville M, Keita A, et al. Prognostic significance of interleukin-17A-producing colorectal tumour antigen-specific T cells. Br J Cancer. 2021;124:1552-1555 pubmed 出版商
Song J, Schwenzer A, Wong A, Turcinov S, Rims C, Martinez L, et al. Shared recognition of citrullinated tenascin-C peptides by T and B cells in rheumatoid arthritis. JCI Insight. 2021;6: pubmed 出版商
Singhania A, Pham J, Dhanwani R, Frazier A, Rezende Dutra J, Marder K, et al. The TCR repertoire of α-synuclein-specific T cells in Parkinson's disease is surprisingly diverse. Sci Rep. 2021;11:302 pubmed 出版商
Lindestam Arlehamn C, Dhanwani R, Pham J, Kuan R, Frazier A, Rezende Dutra J, et al. α-Synuclein-specific T cell reactivity is associated with preclinical and early Parkinson's disease. Nat Commun. 2020;11:1875 pubmed 出版商
Liebowitz D, Gottlieb K, Kolhatkar N, Garg S, Asher J, Nazareno J, et al. Efficacy, immunogenicity, and safety of an oral influenza vaccine: a placebo-controlled and active-controlled phase 2 human challenge study. Lancet Infect Dis. 2020;20:435-444 pubmed 出版商
Schultz H, Østergaard S, Sidney J, Lamberth K, Sette A. The effect of acylation with fatty acids and other modifications on HLA class II:peptide binding and T cell stimulation for three model peptides. PLoS ONE. 2018;13:e0197407 pubmed 出版商
Fostier K, Caers J, Meuleman N, Broos K, Corthals J, Thielemans K, et al. Impact of lenalidomide maintenance on the immune environment of multiple myeloma patients with low tumor burden after autologous stem cell transplantation. Oncotarget. 2018;9:20476-20489 pubmed 出版商
Carrasco Pro S, Lindestam Arlehamn C, Dhanda S, Carpenter C, Lindvall M, Faruqi A, et al. Microbiota epitope similarity either dampens or enhances the immunogenicity of disease-associated antigenic epitopes. PLoS ONE. 2018;13:e0196551 pubmed 出版商
Bieling M, Tischer S, Kalinke U, Blasczyk R, Buus S, Maecker Kolhoff B, et al. Personalized adoptive immunotherapy for patients with EBV-associated tumors and complications: Evaluation of novel naturally processed and presented EBV-derived T-cell epitopes. Oncotarget. 2018;9:4737-4757 pubmed 出版商
Scurr M, Pembroke T, Bloom A, Roberts D, Thomson A, Smart K, et al. Low-Dose Cyclophosphamide Induces Antitumor T-Cell Responses, which Associate with Survival in Metastatic Colorectal Cancer. Clin Cancer Res. 2017;23:6771-6780 pubmed 出版商
Jackson S, Sedikides G, Okecha G, Poole E, Sinclair J, Wills M. Latent Cytomegalovirus (CMV) Infection Does Not Detrimentally Alter T Cell Responses in the Healthy Old, But Increased Latent CMV Carriage Is Related to Expanded CMV-Specific T Cells. Front Immunol. 2017;8:733 pubmed 出版商
Uebele J, Stein C, Nguyen M, Schneider A, Kleinert F, Ticha O, et al. Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus. PLoS Pathog. 2017;13:e1006387 pubmed 出版商
Jackson S, Sedikides G, Mason G, Okecha G, Wills M. Human Cytomegalovirus (HCMV)-Specific CD4+ T Cells Are Polyfunctional and Can Respond to HCMV-Infected Dendritic Cells In Vitro. J Virol. 2017;91: pubmed 出版商
Körber N, Behrends U, Hapfelmeier A, Protzer U, Bauer T. Validation of an IFN?/IL2 FluoroSpot assay for clinical trial monitoring. J Transl Med. 2016;14:175 pubmed 出版商
Kaiser Y, Lepzien R, Kullberg S, Eklund A, Smed Sörensen A, Grunewald J. Expanded lung T-bet+RORγT+ CD4+ T-cells in sarcoidosis patients with a favourable disease phenotype. Eur Respir J. 2016;48:484-94 pubmed 出版商
Brunskole Hummel I, Zitzmann A, Erl M, Wenzel J, Jilg W. Characteristics of immune memory 10-15 years after primary hepatitis B vaccination. Vaccine. 2016;34:636-642 pubmed 出版商
Weinberg A, Muresan P, Richardson K, Fenton T, Domínguez T, Bloom A, et al. Determinants of vaccine immunogenicity in HIV-infected pregnant women: analysis of B and T cell responses to pandemic H1N1 monovalent vaccine. PLoS ONE. 2015;10:e0122431 pubmed 出版商
Janetzki S, Rueger M, Dillenbeck T. Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays. Cells. 2014;3:1102-15 pubmed 出版商
Braesch Andersen S, Paulie S, Smedman C, Mia S, Kumagai Braesch M. ApoE production in human monocytes and its regulation by inflammatory cytokines. PLoS ONE. 2013;8:e79908 pubmed 出版商
Chauvat A, Benhamouda N, Gey A, Lemoine F, Paulie S, Carrat F, et al. Clinical validation of IFN?/IL-10 and IFN?/IL-2 FluoroSpot assays for the detection of Tr1 T cells and influenza vaccine monitoring in humans. Hum Vaccin Immunother. 2014;10:104-13 pubmed 出版商
Sridhar S, Begom S, Bermingham A, Hoschler K, Adamson W, Carman W, et al. Cellular immune correlates of protection against symptomatic pandemic influenza. Nat Med. 2013;19:1305-12 pubmed 出版商
Ohrmalm L, Smedman C, Wong M, Broliden K, Tolfvenstam T, Norbeck O. Decreased functional T lymphocyte-mediated cytokine responses in patients with chemotherapy-induced neutropenia. J Intern Med. 2013;274:363-70 pubmed 出版商
Smedman C, Ernemar T, Gudmundsdotter L, Gille Johnson P, Somell A, Nihlmark K, et al. FluoroSpot Analysis of TLR-Activated Monocytes Reveals Several Distinct Cytokine-Secreting Subpopulations. Scand J Immunol. 2012;75:249-58 pubmed 出版商
产品信息
产品代码 :
FSP-0109-10
产品名称 :
FluoroSpot Plus: Human IFN-gamma/TNF-alpha
Categories :
FluoroSpot Kits
UNSPSC类别 :
Protein chemifluorescent detection reagents or kits or substrates
UNSPSC编码 :
41105331
规格 :
10孔板
规格数目 :
10
尺寸单位 :
plates
2023 USD List :
3885美元
保存期限 :
12 months
Last updated :
2016-04-11
格式 :
pre-coated plates
应用 :
FluoroSpot
Reactivities :
人类
Analytes :
IFNG, TNFA
Recommended Products :
FSP-010209-10
Contents :
BAM-conjugated detection mAb (7-B6-1),Biotinylated detection mAb (MT20D9),Anti-BAM-490,SA-550,Anti-CD3 mAb,Anti-CD28 mAb (CD28-A),FluoroSpot enhancer,Pre-coated FluoroSpot plates (mAbs 1-D1K and MT25C5)
Product Header :
FluoroSpot kit with pre-coated plates for enumeration of cells secreting IFN-γ and TNF-α
描述1 :
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/TNF-α FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting either one or both analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.
描述2 :
Pre-coated plates enable a more rapid assay with minimized variability.
参考文献 :
Thomson, A., et al. (2021). Prognostic significance of interleukin-17A-producing colorectal tumour antigen-specific T cells. British journal of cancer. (http://www.ncbi.nlm.nih.gov/pubmed/33674735) Singhania, A., et al. (2021). The TCR repertoire of alpha-synuclein-specific T cells in Parkinson's disease is surprisingly diverse. Sci Rep. (http://www.ncbi.nlm.nih.gov/pubmed/33432042) Lindestam Arlehamn, C. S., et al. (2020). alpha-Synuclein-specific T cell reactivity is associated with preclinical and early Parkinson's disease. Nat Commun. (http://www.ncbi.nlm.nih.gov/pubmed/32313102) Jackson, S. E., et al. (2017). Latent Cytomegalovirus (CMV) Infection Does Not Detrimentally Alter T Cell Responses in the Healthy Old, But Increased Latent CMV Carriage Is Related to Expanded CMV-Specific T Cells. Front Immunol. (http://www.ncbi.nlm.nih.gov/pubmed/28694811) Bronge, M., et al. (2022). Identification of four novel T cell autoantigens and personal autoreactive profiles in multiple sclerosis. Sci Adv. (http://www.ncbi.nlm.nih.gov/pubmed/35476434)
更多信息或购买 :
公司信息
MABTECH
Box 1233
SE-131 28 Nacka Strand
mabtech@mabtech.com
http://www.mabtech.com
46-8-716-27-00
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