产品简要
公司名称 :
MABTECH
产品类型 :
ELISA/assay
产品名称 :
FluoroSpot Plus: Human IFN-gamma/TNF-alpha
目录 :
FSP-0109-2
规格 :
2孔板
价格 :
890美元
更多信息或购买 :
MABTECH 产品网页
文章摘录数: 20
参考文献
Thomson A, Bento D, Scurr M, Smart K, Somerville M, Keita A, et al. Prognostic significance of interleukin-17A-producing colorectal tumour antigen-specific T cells. Br J Cancer. 2021;124:1552-1555 pubmed 出版商
Foord E, Arruda L, Gaballa A, Klynning C, Uhlin M. Characterization of ascites- and tumor-infiltrating γδ T cells reveals distinct repertoires and a beneficial role in ovarian cancer. Sci Transl Med. 2021;13: pubmed 出版商
Leuchte K, Staib E, Thelen M, Gödel P, Lechner A, Zentis P, et al. Microwave ablation enhances tumor-specific immune response in patients with hepatocellular carcinoma. Cancer Immunol Immunother. 2021;70:893-907 pubmed 出版商
Lindestam Arlehamn C, Dhanwani R, Pham J, Kuan R, Frazier A, Rezende Dutra J, et al. α-Synuclein-specific T cell reactivity is associated with preclinical and early Parkinson's disease. Nat Commun. 2020;11:1875 pubmed 出版商
Schultz H, Østergaard S, Sidney J, Lamberth K, Sette A. The effect of acylation with fatty acids and other modifications on HLA class II:peptide binding and T cell stimulation for three model peptides. PLoS ONE. 2018;13:e0197407 pubmed 出版商
Carrasco Pro S, Lindestam Arlehamn C, Dhanda S, Carpenter C, Lindvall M, Faruqi A, et al. Microbiota epitope similarity either dampens or enhances the immunogenicity of disease-associated antigenic epitopes. PLoS ONE. 2018;13:e0196551 pubmed 出版商
Lund G, Brand S, Ramos T, Jimeno L, Boissy P, Vega F, et al. Strong and frequent T-cell responses to the minor allergen Phl p 12 in Spanish patients IgE-sensitized to Profilins. Allergy. 2018;73:1013-1021 pubmed 出版商
Das A, Rouault Pierre K, Kamdar S, Gomez Tourino I, Wood K, Donaldson I, et al. Adaptive from Innate: Human IFN-γ+CD4+ T Cells Can Arise Directly from CXCL8-Producing Recent Thymic Emigrants in Babies and Adults. J Immunol. 2017;199:1696-1705 pubmed 出版商
Jackson S, Sedikides G, Okecha G, Poole E, Sinclair J, Wills M. Latent Cytomegalovirus (CMV) Infection Does Not Detrimentally Alter T Cell Responses in the Healthy Old, But Increased Latent CMV Carriage Is Related to Expanded CMV-Specific T Cells. Front Immunol. 2017;8:733 pubmed 出版商
Danielzik T, Koldehoff M, Buttkereit U, Beelen D, Horn P, Lindemann M. Sensitive detection of rare antigen-specific T cells directed against Wilms' tumor 1 by FluoroSpot assay. Leuk Lymphoma. 2018;59:490-492 pubmed 出版商
Uebele J, Stein C, Nguyen M, Schneider A, Kleinert F, Ticha O, et al. Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus. PLoS Pathog. 2017;13:e1006387 pubmed 出版商
Jackson S, Sedikides G, Mason G, Okecha G, Wills M. Human Cytomegalovirus (HCMV)-Specific CD4+ T Cells Are Polyfunctional and Can Respond to HCMV-Infected Dendritic Cells In Vitro. J Virol. 2017;91: pubmed 出版商
Clement M, Marsden M, Stacey M, Abdul Karim J, Gimeno Brias S, Costa Bento D, et al. Cytomegalovirus-Specific IL-10-Producing CD4+ T Cells Are Governed by Type-I IFN-Induced IL-27 and Promote Virus Persistence. PLoS Pathog. 2016;12:e1006050 pubmed 出版商
Brunskole Hummel I, Zitzmann A, Erl M, Wenzel J, Jilg W. Characteristics of immune memory 10-15 years after primary hepatitis B vaccination. Vaccine. 2016;34:636-642 pubmed 出版商
Brunskole Hummel I, Huber B, Wenzel J, Jilg W. Markers of Protection in Children and Adolescents Six to Fourteen Years After Primary Hepatitis B Vaccination in Real Life: A Pilot Study. Pediatr Infect Dis J. 2016;35:286-91 pubmed 出版商
Dillenbeck T, Gelius E, Fohlstedt J, Ahlborg N. Triple Cytokine FluoroSpot Analysis of Human Antigen-Specific IFN-γ, IL-17A and IL-22 Responses. Cells. 2014;3:1116-30 pubmed 出版商
Han W, van Twillert I, Poelen M, Helm K, van de Kassteele J, Verheij T, et al. Loss of multi-epitope specificity in memory CD4(+) T cell responses to B. pertussis with age. PLoS ONE. 2013;8:e83583 pubmed 出版商
Braesch Andersen S, Paulie S, Smedman C, Mia S, Kumagai Braesch M. ApoE production in human monocytes and its regulation by inflammatory cytokines. PLoS ONE. 2013;8:e79908 pubmed 出版商
Smedman C, Ernemar T, Gudmundsdotter L, Gille Johnson P, Somell A, Nihlmark K, et al. FluoroSpot Analysis of TLR-Activated Monocytes Reveals Several Distinct Cytokine-Secreting Subpopulations. Scand J Immunol. 2012;75:249-58 pubmed 出版商
Lee F, Walsh E, Falsey A, Lumb M, Okam N, Liu N, et al. Human infant respiratory syncytial virus (RSV)-specific type 1 and 2 cytokine responses ex vivo during primary RSV infection. J Infect Dis. 2007;195:1779-88 pubmed
产品信息
产品代码 :
FSP-0109-2
产品名称 :
FluoroSpot Plus: Human IFN-gamma/TNF-alpha
Categories :
FluoroSpot Kits
UNSPSC类别 :
Protein chemifluorescent detection reagents or kits or substrates
UNSPSC编码 :
41105331
规格 :
2孔板
规格数目 :
2
尺寸单位 :
plates
2023 USD List :
890美元
保存期限 :
12 months
Last updated :
2016-04-11
格式 :
pre-coated plates
应用 :
FluoroSpot
Reactivities :
人类
Analytes :
IFNG, TNFA
Recommended Products :
FSP-010209-2
Contents :
BAM-conjugated detection mAb (7-B6-1),Biotinylated detection mAb (MT20D9),Anti-BAM-490,SA-550,Anti-CD3 mAb,Anti-CD28 mAb (CD28-A),FluoroSpot enhancer,Pre-coated FluoroSpot plates (mAbs 1-D1K and MT25C5)
Product Header :
FluoroSpot kit with pre-coated plates for enumeration of cells secreting IFN-γ and TNF-α
描述1 :
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/TNF-α FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting either one or both analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.
描述2 :
Pre-coated plates enable a more rapid assay with minimized variability.
参考文献 :
Thomson, A., et al. (2021). Prognostic significance of interleukin-17A-producing colorectal tumour antigen-specific T cells. British journal of cancer. (http://www.ncbi.nlm.nih.gov/pubmed/33674735) Leuchte, K., et al. (2021). Microwave ablation enhances tumor-specific immune response in patients with hepatocellular carcinoma. Cancer immunology, immunotherapy : CII. (http://www.ncbi.nlm.nih.gov/pubmed/33006650) Brunskole Hummel, I., et al. (2016). Markers of Protection in Children and Adolescents Six to Fourteen Years After Primary Hepatitis B Vaccination in Real Life. The Pediatric Infectious Disease Journal. (http://www.ncbi.nlm.nih.gov/pubmed/26569192) Schultz, H. S., et al. (2018). The effect of acylation with fatty acids and other modifications on HLA class II:peptide binding and T cell stimulation for three model peptides. PLoS ONE. (http://www.ncbi.nlm.nih.gov/pubmed/29758051) Das, A., et al. (2017). Adaptive from Innate: Human IFN-gamma(+)CD4(+) T Cells Can Arise Directly from CXCL8-Producing Recent Thymic Emigrants in Babies and Adults. Journal of immunology. (http://www.ncbi.nlm.nih.gov/pubmed/28754679)
更多信息或购买 :
MABTECH 产品网页
公司信息
MABTECH
Box 1233
SE-131 28 Nacka Strand
mabtech@mabtech.com
http://www.mabtech.com
46-8-716-27-00
公司总部: 瑞典