Leelatian N, Sinnaeve J, Mistry A, Barone S, Brockman A, Diggins K, et al. Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells. elife. 2020;9: pubmed 出版商

Katsuda T, Matsuzaki J, Yamaguchi T, Yamada Y, Prieto Vila M, Hosaka K, et al. Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes. elife. 2019;8: pubmed 出版商

Vazquez Iglesias L, Barcia Castro L, Rodríguez Quiroga M, Páez de la Cadena M, Rodríguez Berrocal J, Cordero O. Surface expression marker profile in colon cancer cell lines and sphere-derived cells suggests complexity in CD26+ cancer stem cells subsets. Biol Open. 2019;8: pubmed 出版商

Ong D, Hu B, Ho Y, Sauvé C, Bristow C, Wang Q, et al. PAF promotes stemness and radioresistance of glioma stem cells. Proc Natl Acad Sci U S A. 2017;114:E9086-E9095 pubmed 出版商

Vernot J, Bonilla X, Rodriguez Pardo V, Vanegas N. Phenotypic and Functional Alterations of Hematopoietic Stem and Progenitor Cells in an In Vitro Leukemia-Induced Microenvironment. Int J Mol Sci. 2017;18: pubmed 出版商

Fromm J, Thomas A, Wood B. Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting. Am J Pathol. 2017;187:304-317 pubmed 出版商

Sun H, Zhang M, Cheng K, Li P, Han S, Li R, et al. Resistance of glioma cells to nutrient-deprived microenvironment can be enhanced by CD133-mediated autophagy. Oncotarget. 2016;7:76238-76249 pubmed 出版商

Sousa A, Rei M, Freitas R, Ricardo S, Caffrey T, David L, et al. Effect of MUC1/?-catenin interaction on the tumorigenic capacity of pancreatic CD133+ cells. Oncol Lett. 2016;12:1811-1817 pubmed

Nel I, Gauler T, Bublitz K, Lazaridis L, Goergens A, Giebel B, et al. Circulating Tumor Cell Composition in Renal Cell Carcinoma. PLoS ONE. 2016;11:e0153018 pubmed 出版商

Garcia C, Videla Richardson G, Dimopoulos N, Fernandez Espinosa D, Miriuka S, Sevlever G, et al. Human Pluripotent Stem Cells and Derived Neuroprogenitors Display Differential Degrees of Susceptibility to BH3 Mimetics ABT-263, WEHI-539 and ABT-199. PLoS ONE. 2016;11:e0152607 pubmed 出版商

Gradiz R, Silva H, Carvalho L, Botelho M, Mota Pinto A. MIA PaCa-2 and PANC-1 - pancreas ductal adenocarcinoma cell lines with neuroendocrine differentiation and somatostatin receptors. Sci Rep. 2016;6:21648 pubmed 出版商

Acikgoz E, Guven U, Duzagac F, Uslu R, Kara M, Soner B, et al. Enhanced G2/M Arrest, Caspase Related Apoptosis and Reduced E-Cadherin Dependent Intercellular Adhesion by Trabectedin in Prostate Cancer Stem Cells. PLoS ONE. 2015;10:e0141090 pubmed 出版商

Sipos F, Constantinovits M, Valcz G, Tulassay Z, Műzes G. Association of hepatocyte-derived growth factor receptor/caudal type homeobox 2 co-expression with mucosal regeneration in active ulcerative colitis. World J Gastroenterol. 2015;21:8569-79 pubmed 出版商

Russell R, Perkhofer L, Liebau S, Lin Q, Lechel A, Feld F, et al. Loss of ATM accelerates pancreatic cancer formation and epithelial-mesenchymal transition. Nat Commun. 2015;6:7677 pubmed 出版商

Bongiorno Borbone L, Giacobbe A, Compagnone M, Eramo A, De Maria R, Peschiaroli A, et al. Anti-tumoral effect of desmethylclomipramine in lung cancer stem cells. Oncotarget. 2015;6:16926-38 pubmed

Cioffi M, D Alterio C, Camerlingo R, Tirino V, Consales C, Riccio A, et al. Identification of a distinct population of CD133(+)CXCR4(+) cancer stem cells in ovarian cancer. Sci Rep. 2015;5:10357 pubmed 出版商

Nunukova A, Neradil J, Skoda J, Jaroš J, Hampl A, Sterba J, et al. Atypical nuclear localization of CD133 plasma membrane glycoprotein in rhabdomyosarcoma cell lines. Int J Mol Med. 2015;36:65-72 pubmed 出版商

Flores Nascimento M, Aléssio A, de Andrade Orsi F, Annichino Bizzacchi J. CD144, CD146 and VEGFR-2 properly identify circulating endothelial cell. Rev Bras Hematol Hemoter. 2015;37:98-102 pubmed 出版商

Chavali P, Saini R, Zhai Q, Vizlin Hodzic D, Venkatabalasubramanian S, Hayashi A, et al. TLX activates MMP-2, promotes self-renewal of tumor spheres in neuroblastoma and correlates with poor patient survival. Cell Death Dis. 2014;5:e1502 pubmed 出版商

Cucak H, Vistisen D, Witte D, Philipsen A, Rosendahl A. Reduction of specific circulating lymphocyte populations with metabolic risk factors in patients at risk to develop type 2 diabetes. PLoS ONE. 2014;9:e107140 pubmed 出版商

Holmberg Olausson K, Maire C, Haidar S, Ling J, Learner E, Nistér M, et al. Prominin-1 (CD133) defines both stem and non-stem cell populations in CNS development and gliomas. PLoS ONE. 2014;9:e106694 pubmed 出版商

Wennerström A, Lothe I, Sandhu V, Kure E, Myklebost O, Munthe E. Generation and characterisation of novel pancreatic adenocarcinoma xenograft models and corresponding primary cell lines. PLoS ONE. 2014;9:e103873 pubmed 出版商

Jeon H, Kim S, Jin X, Park J, Kim S, Joshi K, et al. Crosstalk between glioma-initiating cells and endothelial cells drives tumor progression. Cancer Res. 2014;74:4482-92 pubmed 出版商

Yu P, Yan M, Lai H, Huang R, Chou Y, Lin W, et al. Downregulation of miR-29 contributes to cisplatin resistance of ovarian cancer cells. Int J Cancer. 2014;134:542-51 pubmed 出版商

Denecke B, Horsch L, Radtke S, Fischer J, Horn P, Giebel B. Human endothelial colony-forming cells expanded with an improved protocol are a useful endothelial cell source for scaffold-based tissue engineering. J Tissue Eng Regen Med. 2015;9:E84-97 pubmed 出版商

Takeda T, Yokoyama Y, Takahashi H, Okuzaki D, Asai K, Itakura H, et al. A stem cell marker KLF5 regulates CCAT1 via three-dimensional genome structure in colorectal cancer cells. Br J Cancer. 2022;126:109-119 pubmed 出版商

Ganesh K, Basnet H, Kaygusuz Y, Laughney A, He L, Sharma R, et al. L1CAM defines the regenerative origin of metastasis-initiating cells in colorectal cancer. Nat Cancer. 2020;1:28-45 pubmed 出版商

Veschi V, Mangiapane L, Nicotra A, Di Franco S, Scavo E, Apuzzo T, et al. Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant. Oncogene. 2020;39:987-1003 pubmed 出版商

Wang Y, Yin K, Tian J, Xia X, Ma J, Tang X, et al. Granulocytic Myeloid-Derived Suppressor Cells Promote the Stemness of Colorectal Cancer Cells through Exosomal S100A9. Adv Sci (Weinh). 2019;6:1901278 pubmed 出版商

Fakiruddin K, Lim M, Nordin N, Rosli R, Zakaria Z, Abdullah S. Targeting of CD133+ Cancer Stem Cells by Mesenchymal Stem Cell Expressing TRAIL Reveals a Prospective Role of Apoptotic Gene Regulation in Non-Small Cell Lung Cancer. Cancers (Basel). 2019;11: pubmed 出版商

Klotz B, Oosterhoff L, Utomo L, Lim K, Vallmajo Martin Q, Clevers H, et al. A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues. Adv Healthc Mater. 2019;8:e1900979 pubmed 出版商

Essex A, Pineda J, Acharya G, Xin H, Evans J, Iorns E, et al. Replication Study: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment. elife. 2019;8: pubmed 出版商

Fujino S, Miyoshi N. Oct4 Gene Expression in Primary Colorectal Cancer Promotes Liver Metastasis. Stem Cells Int. 2019;2019:7896524 pubmed 出版商

Atlasy N, Amidi F, Mortezaee K, Fazeli M, Mowla S, Malek F. Expression Patterns for TETs, LGR5 and BMI1 in Cancer Stem-like Cells Isolated from Human Colon Cancer. Avicenna J Med Biotechnol. 2019;11:156-161 pubmed

White Gilbertson S, Lu P, Norris J, Voelkel Johnson C. Genetic and pharmacological inhibition of acid ceramidase prevents asymmetric cell division by neosis. J Lipid Res. 2019;: pubmed 出版商

Kim T, Ko Y, Ha S, Lee H. Impact of in vitro driven expression signatures of CD133 stem cell marker and tumor stroma on clinical outcomes in gastric cancers. BMC Cancer. 2019;19:119 pubmed 出版商

Taracanova A, Tsilioni I, Conti P, Norwitz E, Leeman S, Theoharides T. Substance P and IL-33 administered together stimulate a marked secretion of IL-1β from human mast cells, inhibited by methoxyluteolin. Proc Natl Acad Sci U S A. 2018;115:E9381-E9390 pubmed 出版商

Chawla A, Cororaton A, Idowu M, Damle P, Szomju B, Ellis K, et al. An intestinal stem cell niche in Apc mutated neoplasia targetable by CtBP inhibition. Oncotarget. 2018;9:32408-32418 pubmed 出版商

Lenos K, Miedema D, Lodestijn S, Nijman L, van den Bosch T, Romero Ros X, et al. Stem cell functionality is microenvironmentally defined during tumour expansion and therapy response in colon cancer. Nat Cell Biol. 2018;20:1193-1202 pubmed 出版商

Maiuthed A, Bhummaphan N, Luanpitpong S, Mutirangura A, Aporntewan C, Meeprasert A, et al. Nitric oxide promotes cancer cell dedifferentiation by disrupting an Oct4:caveolin-1 complex: A new regulatory mechanism for cancer stem cell formation. J Biol Chem. 2018;293:13534-13552 pubmed 出版商

Glumac P, LeBeau A. The role of CD133 in cancer: a concise review. Clin Transl Med. 2018;7:18 pubmed 出版商

Wang X, Huang Z, Wu Q, Prager B, Mack S, Yang K, et al. MYC-Regulated Mevalonate Metabolism Maintains Brain Tumor-Initiating Cells. Cancer Res. 2017;77:4947-4960 pubmed 出版商

Taracanova A, Alevizos M, Karagkouni A, Weng Z, Norwitz E, Conti P, et al. SP and IL-33 together markedly enhance TNF synthesis and secretion from human mast cells mediated by the interaction of their receptors. Proc Natl Acad Sci U S A. 2017;114:E4002-E4009 pubmed 出版商

Zhuang Z, Xie N, Hu J, Yu P, Wang C, Hu X, et al. Interplay between ΔNp63 and miR-138-5p regulates growth, metastasis and stemness of oral squamous cell carcinoma. Oncotarget. 2017;8:21954-21973 pubmed 出版商

Cervantes Madrid D, Wettergren Y, Falk P, Lundholm K, Asting A. DNA alterations in Cd133+ and Cd133- tumour cells enriched from intra-operative human colon tumour biopsies. BMC Cancer. 2017;17:219 pubmed 出版商

Lee J, Snyder E, Liu Y, Gu X, Wang J, Flowers B, et al. Reconstituting development of pancreatic intraepithelial neoplasia from primary human pancreas duct cells. Nat Commun. 2017;8:14686 pubmed 出版商

Song Y, Jang J, Shin T, Bae S, Kim J, Kim K, et al. Sulfasalazine attenuates evading anticancer response of CD133-positive hepatocellular carcinoma cells. J Exp Clin Cancer Res. 2017;36:38 pubmed 出版商

Wang M, Han J, Marcar L, Black J, Liu Q, Li X, et al. Radiation Resistance in KRAS-Mutated Lung Cancer Is Enabled by Stem-like Properties Mediated by an Osteopontin-EGFR Pathway. Cancer Res. 2017;77:2018-2028 pubmed 出版商

Bijangi Vishehsaraei K, Reza Saadatzadeh M, Wang H, Nguyen A, Kamocka M, Cai W, et al. Sulforaphane suppresses the growth of glioblastoma cells, glioblastoma stem cell-like spheroids, and tumor xenografts through multiple cell signaling pathways. J Neurosurg. 2017;127:1219-1230 pubmed 出版商

Liau B, Sievers C, Donohue L, Gillespie S, Flavahan W, Miller T, et al. Adaptive Chromatin Remodeling Drives Glioblastoma Stem Cell Plasticity and Drug Tolerance. Cell Stem Cell. 2017;20:233-246.e7 pubmed 出版商

Li R, He Q, Han S, Zhang M, Liu J, Su M, et al. MBD3 inhibits formation of liver cancer stem cells. Oncotarget. 2017;8:6067-6078 pubmed 出版商

Frasson C, Rampazzo E, Accordi B, Beggio G, Pistollato F, Basso G, et al. Inhibition of PI3K Signalling Selectively Affects Medulloblastoma Cancer Stem Cells. Biomed Res Int. 2015;2015:973912 pubmed 出版商

Sun Y, Song G, Sun N, Chen J, Yang S. Slug overexpression induces stemness and promotes hepatocellular carcinoma cell invasion and metastasis. Oncol Lett. 2014;7:1936-1940 pubmed

Pitule P, Cedíková M, Daum O, Vojtisek J, Vycital O, Hosek P, et al. Immunohistochemical detection of cancer stem cell related markers CD44 and CD133 in metastatic colorectal cancer patients. Biomed Res Int. 2014;2014:432139 pubmed 出版商

Schiffer D, Mellai M, Annovazzi L, Caldera V, Piazzi A, Denysenko T, et al. Stem cell niches in glioblastoma: a neuropathological view. Biomed Res Int. 2014;2014:725921 pubmed 出版商

Tsang H, Leiper J, Hou Lao K, Dowsett L, Delahaye M, Barnes G, et al. Role of asymmetric methylarginine and connexin 43 in the regulation of pulmonary endothelial function. Pulm Circ. 2013;3:675-91 pubmed 出版商

Hu Y, Ru N, Xiao H, Chaturbedi A, Hoa N, Tian X, et al. Tumor-specific chromosome mis-segregation controls cancer plasticity by maintaining tumor heterogeneity. PLoS ONE. 2013;8:e80898 pubmed 出版商

Steinmetz N, Maurer J, Sheng H, Bensussan A, Maricic I, Kumar V, et al. Two Domains of Vimentin Are Expressed on the Surface of Lymph Node, Bone and Brain Metastatic Prostate Cancer Lines along with the Putative Stem Cell Marker Proteins CD44 and CD133. Cancers (Basel). 2011;3:2870-85 pubmed 出版商

Liu H, Zhang W, Jia Y, Yu Q, Grau G, Peng L, et al. Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells. Cell Death Dis. 2013;4:e857 pubmed 出版商

Jelen S, Parm Ulhøi B, Larsen A, Frøkiær J, Nielsen S, Rutzler M. AQP9 expression in glioblastoma multiforme tumors is limited to a small population of astrocytic cells and CD15(+)/CalB(+) leukocytes. PLoS ONE. 2013;8:e75764 pubmed 出版商

Rheinbay E, Suvà M, Gillespie S, Wakimoto H, Patel A, Shahid M, et al. An aberrant transcription factor network essential for Wnt signaling and stem cell maintenance in glioblastoma. Cell Rep. 2013;3:1567-79 pubmed 出版商

Ye X, Wang H, Zhou J, Li H, Liu J, Wang Z, et al. The effect of Heparin-VEGF multilayer on the biocompatibility of decellularized aortic valve with platelet and endothelial progenitor cells. PLoS ONE. 2013;8:e54622 pubmed 出版商

Walton R, Parmentier T, Wolfe J. Postnatal neural precursor cell regions in the rostral subventricular zone, hippocampal subgranular zone and cerebellum of the dog (Canis lupus familiaris). Histochem Cell Biol. 2013;139:415-29 pubmed 出版商

Czyz M, Koprowska K, Sztiller Sikorska M. Parthenolide reduces the frequency of ABCB5-positive cells and clonogenic capacity of melanoma cells from anchorage independent melanospheres. Cancer Biol Ther. 2013;14:135-45 pubmed 出版商

Fredebohm J, Boettcher M, Eisen C, Gaida M, Heller A, Keleg S, et al. Establishment and characterization of a highly tumourigenic and cancer stem cell enriched pancreatic cancer cell line as a well defined model system. PLoS ONE. 2012;7:e48503 pubmed 出版商

Wong C, Namdarian B, Chua J, Chin X, Speirs R, Nguyen T, et al. Levels of a subpopulation of platelets, but not circulating endothelial cells, predict early treatment failure in prostate cancer patients after prostatectomy. Br J Cancer. 2012;107:1564-73 pubmed 出版商

Ferro F, Spelat R, D Aurizio F, Puppato E, Pandolfi M, Beltrami A, et al. Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics. PLoS ONE. 2012;7:e41774 pubmed 出版商

Clark P, Iida M, Treisman D, Kalluri H, Ezhilan S, Zorniak M, et al. Activation of multiple ERBB family receptors mediates glioblastoma cancer stem-like cell resistance to EGFR-targeted inhibition. Neoplasia. 2012;14:420-8 pubmed

Park T, Zimmerlin L, Zambidis E. Efficient and simultaneous generation of hematopoietic and vascular progenitors from human induced pluripotent stem cells. Cytometry A. 2013;83:114-26 pubmed 出版商

Qi Y, Qian L, Sun B, Liu L, Wu P, Sun L. Inhaled NO contributes to lung repair in piglets with acute respiratory distress syndrome via increasing circulating endothelial progenitor cells. PLoS ONE. 2012;7:e33859 pubmed 出版商

Mak A, Blakely K, Williams R, Penttilä P, Shukalyuk A, Osman K, et al. CD133 protein N-glycosylation processing contributes to cell surface recognition of the primitive cell marker AC133 epitope. J Biol Chem. 2011;286:41046-56 pubmed 出版商

Fusi A, Reichelt U, Busse A, Ochsenreither S, Rietz A, Maisel M, et al. Expression of the stem cell markers nestin and CD133 on circulating melanoma cells. J Invest Dermatol. 2011;131:487-94 pubmed 出版商

Estes M, Mund J, Mead L, Prater D, Cai S, Wang H, et al. Application of polychromatic flow cytometry to identify novel subsets of circulating cells with angiogenic potential. Cytometry A. 2010;77:831-9 pubmed 出版商

Fonseca A, Freund D, Bornhauser M, Corbeil D. Polarization and migration of hematopoietic stem and progenitor cells rely on the RhoA/ROCK I pathway and an active reorganization of the microtubule network. J Biol Chem. 2010;285:31661-71 pubmed 出版商

Behbod F, Kittrell F, LaMarca H, Edwards D, Kerbawy S, Heestand J, et al. An intraductal human-in-mouse transplantation model mimics the subtypes of ductal carcinoma in situ. Breast Cancer Res. 2009;11:R66 pubmed 出版商

Metsuyanim S, Harari Steinberg O, Buzhor E, Omer D, Pode Shakked N, Ben Hur H, et al. Expression of stem cell markers in the human fetal kidney. PLoS ONE. 2009;4:e6709 pubmed 出版商

Margolis D, Morris L, Papadopoulos M, Weinberg L, Filip J, Lang S, et al. Phase I study of H5.020CMV.PDGF-beta to treat venous leg ulcer disease. Mol Ther. 2009;17:1822-9 pubmed 出版商

Maxwell S, Li Z, Jaye D, Jaya D, Ballard S, Ferrell J, et al. 14-3-3zeta mediates resistance of diffuse large B cell lymphoma to an anthracycline-based chemotherapeutic regimen. J Biol Chem. 2009;284:22379-89 pubmed 出版商

Lin L, Amin R, Gallicano G, Glasgow E, Jogunoori W, Jessup J, et al. The STAT3 inhibitor NSC 74859 is effective in hepatocellular cancers with disrupted TGF-beta signaling. Oncogene. 2009;28:961-72 pubmed 出版商

Immervoll H, Hoem D, Sakariassen P, Steffensen O, Molven A. Expression of the "stem cell marker" CD133 in pancreas and pancreatic ductal adenocarcinomas. BMC Cancer. 2008;8:48 pubmed 出版商

Alvarez D, Huang L, King J, ElZarrad M, Yoder M, Stevens T. Lung microvascular endothelium is enriched with progenitor cells that exhibit vasculogenic capacity. Am J Physiol Lung Cell Mol Physiol. 2008;294:L419-30 pubmed

Duda D, Cohen K, Scadden D, Jain R. A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood. Nat Protoc. 2007;2:805-10 pubmed

Galic Z, Kitchen S, Kacena A, Subramanian A, Burke B, Cortado R, et al. T lineage differentiation from human embryonic stem cells. Proc Natl Acad Sci U S A. 2006;103:11742-7 pubmed

Cummings B, Uchida N, Tamaki S, Salazar D, Hooshmand M, Summers R, et al. Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice. Proc Natl Acad Sci U S A. 2005;102:14069-74 pubmed

Bussolati B, Bruno S, Grange C, Buttiglieri S, Deregibus M, Cantino D, et al. Isolation of renal progenitor cells from adult human kidney. Am J Pathol. 2005;166:545-55 pubmed

Kogler G, Sensken S, Airey J, Trapp T, MUSCHEN M, Feldhahn N, et al. A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential. J Exp Med. 2004;200:123-35 pubmed

Giebel B, Corbeil D, Beckmann J, Höhn J, Freund D, Giesen K, et al. Segregation of lipid raft markers including CD133 in polarized human hematopoietic stem and progenitor cells. Blood. 2004;104:2332-8 pubmed

Richardson G, Robson C, Lang S, Neal D, Maitland N, Collins A. CD133, a novel marker for human prostatic epithelial stem cells. J Cell Sci. 2004;117:3539-45 pubmed

Ingram D, Mead L, Tanaka H, Meade V, Fenoglio A, Mortell K, et al. Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood. Blood. 2004;104:2752-60 pubmed

Wagner W, Ansorge A, Wirkner U, Eckstein V, Schwager C, Blake J, et al. Molecular evidence for stem cell function of the slow-dividing fraction among human hematopoietic progenitor cells by genome-wide analysis. Blood. 2004;104:675-86 pubmed

Piechaczek C. CD133. J Biol Regul Homeost Agents. 2001;15:101-2 pubmed

Uchida N, Buck D, He D, Reitsma M, Masek M, Phan T, et al. Direct isolation of human central nervous system stem cells. Proc Natl Acad Sci U S A. 2000;97:14720-5 pubmed

Gehling U, Ergun S, Schumacher U, Wagener C, Pantel K, Otte M, et al. In vitro differentiation of endothelial cells from AC133-positive progenitor cells. Blood. 2000;95:3106-12 pubmed

Gallacher L, Murdoch B, Wu D, Karanu F, Keeney M, Bhatia M. Isolation and characterization of human CD34(-)Lin(-) and CD34(+)Lin(-) hematopoietic stem cells using cell surface markers AC133 and CD7. Blood. 2000;95:2813-20 pubmed

Peichev M, Naiyer A, Pereira D, Zhu Z, Lane W, Williams M, et al. Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors. Blood. 2000;95:952-8 pubmed

Buhring H, Seiffert M, Bock T, Scheding S, Thiel A, Scheffold A, et al. Expression of novel surface antigens on early hematopoietic cells. Ann N Y Acad Sci. 1999;872:25-38; discussion 38-9 pubmed