β微管蛋白
MyBioSource
目录: MBS470004
小鼠 单克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格: 0.1毫升
价格: 170美元
至产商
Chicken Polyclonal (IgY) to Mouse TUBB3 / Tubulin Beta 3
MyBioSource
目录: MBS244264
鸡 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
规格: 0.075毫克
价格: 495美元
至产商
兔抗人类微管蛋白, 乙3多克隆抗体
MyBioSource
目录: MBS712419
兔 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 酶免疫法
规格: 0.05毫升
价格: 375美元
至产商
抗乙III微管蛋白兔单克隆抗体
MyBioSource
目录: MBS179753
兔 单克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 流式细胞仪

Immunohistochemical analysis of paraffin-embedded human thyroid cancer, using beta III Tubulin Antibody(MBS179753). TUBB3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TUBB3 Antibody (MBS179753)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of beta III Tubulin expression in (1) HeLa cell lysate; (2) PC-12 cell lysate; (3) Mouse brain lysate; (4) Rat brain lysate with beta Tubulin III Antibody (MBS179753). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TUBB3
规格: 0.03毫克
价格: 160美元
至产商
Chicken Polyclonal (IgY) to Human TUBB3 / Tubulin Beta 3
MyBioSource
目录: MBS247962
鸡 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学

Anti-Tuj1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
规格: 0.05毫升
价格: 530美元
至产商