大鼠 单克隆 (NIMR-4)
反应物种: 人类, 小鼠
应用: 流式细胞仪
反应物种: 人类, 小鼠
应用: 流式细胞仪
大鼠 单克隆 (YE2/36 HLK)
反应物种: 人类
共轭标签: 生物素
应用: 免疫组化
反应物种: 人类
共轭标签: 生物素
应用: 免疫组化
大鼠 单克隆 (YE2/36 HLK)
反应物种: 人类
应用: 免疫组化, 流式细胞仪, 流式细胞仪
反应物种: 人类
应用: 免疫组化, 流式细胞仪, 流式细胞仪
大鼠 单克隆 ([YE2/36-HLK])
反应物种: 人类
共轭标签: 异硫氰酸荧光素
应用: 流式细胞仪, 流式细胞仪
反应物种: 人类
共轭标签: 异硫氰酸荧光素
应用: 流式细胞仪, 流式细胞仪
Published customer image: Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometryImage caption:BILF1 identified as a lytic gene that downregulates surface MHC class I. 293 (A) or MJS (B) cells were transfected with different EBV genes in the bi-cistronic vector, pCDNA3-IRES-nlsGFP. At 48 hr post-transfection, surface MHC class I was stained with PE-conjugated W6/32 mAb and (in MJS only) MHC class II was stained with PE-conjugated anti-DR mAb, YE2/36-HLK. Two-colour flow cytometry was used to analyse staining in the untransfected GFP- population, shown as the solid line histogram, and in the transfected GFP+ population, shown as the dashed line histogram. The grey histogram denotes background staining obtained with an isotype control PE-conjugated antibody.From: Zuo J, Currin A, Griffin BD, Shannon-Lowe C, Thomas WA, et al. (2009) The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation. PLoS Pathog 5(1): e1000255.
Published customer image: Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometryImage caption:BILF1 downregulates surface MHC class I expression and inhibits the T cell recognition of endogenous EBV antigen in LCLs. (A) LCLs were transduced with PLZRS-HABILF1-IRES-GFP retrovirus. After 6 days, surface MHC class I was stained with PE-conjugated W6/32 mAb and MHC class II was stained with PE-conjugated anti-DR mAb, YE2/36-HLK. Two-colour flow cytometry was used to analyze staining in the untransduced, GFP-, population, shown as the solid line histogram, and in the transduced GFP+ (BILF1+) population, shown as the dashed line histogram. The grey histogram denotes background staining obtained with an isotype control PE-conjugated antibody. (B) LCL cultures transduced with control retrovirus or with the BILF1 retrovirus were sorted by flow cytometry to generate GFP+/BILF1- and GFP+/BILF1+ lines to use as targets in assays with EBV-specific T cells. The control and BILF1+ LCL targets were incubated with HLA-matched CD8+ effector T cells clones specific for EBNA1 (HPV), EBNA3A (YPL), or LMP2A (CLG) peptides, or a CD4+ effector T cell clone specific for a BHRF1 (PYY) peptide. After 18 hrs the supernatants were tested for the release of IFN-? as a measure of T cell recognition. All results are expressed as IFN-? release in pg/ml and error bars indicate standard deviation of triplicate cultures.From: Zuo J, Currin A, Griffin BD, Shannon-Lowe C, Thomas WA, et al. (2009) The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation. PLoS Pathog 5(1): e1000255.
Published customer image: Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometryImage caption:HLA-DR positive monocytes. The percentage HLA-DR positive monocytes was decreased in all patients compared to controls (P = 0.002). The pre-operative ( black square ) lowest percentage was seen in patients who developed respiratory failure (P = 0.002). Eighteen hours after intramedullary nailing ( open triangle ), a further decrease in HLA-DR positive monocytes was seen in patients with isolated femur fracture (P < 0.001) and multitrauma patients (P = 0.047).From: Hietbrink, Falco, Leo Koenderman, and Luke PH Leenen. œIntramedullary Nailing of the Femur and the Systemic Activation of Monocytes and Neutrophils. World Journal of Emergency Surgery?: WJES 6 (2011): 34. PMC. Web. 22 Jan. 2015.
规格: 0.1毫克
价格: 370美元
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