小鼠 单克隆 (10T1)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫沉淀, 酶免疫法
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫沉淀, 酶免疫法
小鼠 单克隆 (P10T1AT)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 酶免疫法
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 酶免疫法
小鼠 单克隆 (10T1)
反应物种: 人类, 小鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 酶免疫法
反应物种: 人类, 小鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 酶免疫法
兔 单克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 流式细胞仪
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 流式细胞仪
Western blot analysis of IRF5 expression in THP-1 cell lysate (MBS179503). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IRF5
规格: 0.03毫克
价格: 160美元
至产商
兔 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
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