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兔抗CD4多克隆
MyBioSource
目录: MBS551067
兔 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
酶免疫法
规格: 0.1毫克
价格: 280美元
至产商
Anti-CD3 epsilon antibody
MyBioSource
目录: MBS355170
兔 多克隆
反应物种:
人类
,
小鼠
,
狗
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
,
免疫组化-石蜡切片
规格: 0.1毫克
价格: 285美元
至产商
Anti-CD3 epsilon Rabbit Monoclonal Antibody
MyBioSource
目录: MBS179879
兔 单克隆 ([EED-3])
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫沉淀
,
流式细胞仪
,
流式细胞仪
Western blot analysis of CD3 epsilon expression in Jurkat cell lysate (MBS179879). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3E monoclonal antibody overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD3E.
IHC analysis of CD3 epsilon using anti- CD3 epsilon antibody (MBS179879) on human colon cancer. CD3 epsilon was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- CD3 epsilon Antibody (MBS179879) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (MBS176451) with DAB as the chromogen.
规格: 0.03毫克
价格: 160美元
至产商
CD3E抗体
MyBioSource
目录: MBS200286
小鼠 单克隆 ([AT3H3])
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫细胞化学
,
流式细胞仪
,
流式细胞仪
,
酶免疫法
Fig. 1: The extract of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Fig. 2: The Human Recombinant protein CD3E (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
ICC/IF analysis of CD3E in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CD3E antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow cytometry analysis of CD3E in Jurkat cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
规格: 0.05毫升
价格: 240美元
至产商
CD3e(T细胞标记)小鼠单克隆抗体[克隆C3e/1308]
MyBioSource
目录: MBS4380150
小鼠 单克隆 ([C3e/1308])
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
,
流式细胞仪
,
流式细胞仪
Western Blot of recombinant CD3e and Jurkat Cell Lysate using CD3e Monoclonal Antibody (C3e/1308)
SDS-PAGE Analysis Purified CD3 epsilon Monoclonal Antibody (C3e/1308). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of human Jurkat cell lysate using CD3e Monoclonal Antibody (C3e/1308).
规格: 0.02 mg (With BSA & Azide at 0.2 mg/ml)
价格: 190美元
至产商
CD3e(T细胞标记)
MyBioSource
目录: MBS438059
兔 多克隆
反应物种:
人类
,
小鼠
,
大鼠
,
狗
,
鸡
,
牛
,
黑猩猩
,
猕猴
应用:
免疫印迹
,
免疫细胞化学
,
免疫沉淀
,
流式细胞仪
,
流式细胞仪
Western blot of CD3e in human Jurkat cells . 1) absence . 2) presence of immunizing peptide. 3) Mouse thymus probed with CD3e Rabbit PAb.
Western Blot of lurkat Cell Lysate using CD3e Rabbit Polyclonal Antibody.
规格: 0.02 mg (With BSA & Azide at 0.2 mg/ml)
价格: 190美元
至产商
CD3(T细胞标记)小鼠单克隆抗体
MyBioSource
目录: MBS439364
小鼠 单克隆 ([RIV9])
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫细胞化学
,
流式细胞仪
,
流式细胞仪
SDS-PAGE Analysis. CD3e Mouse Monoclonal Antibody (RIV9). Confirmation of Integrity and Purity of Antibody
Flow Cytometric Analysis of Jurkat Cells using CD3e Mouse Monoclonal Antibody (RIV9). Followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)
规格: 0.02 mg (With BSA & Azide at 0.2 mg/ml)
价格: 190美元
至产商
兔抗CD3多克隆
MyBioSource
目录: MBS551029
兔 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
酶免疫法
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of FFPE rat kidney sections showing lymphocyte expression of CD3. It was used as primary antibody, dilution 1:200, DAB chromogenic staining.
Immunoflourescence of human colon cancer sections showing expression of CD3. MBS551029 was used as a primary antibody , dilution 1:60.
规格: 0.1毫克
价格: 280美元
至产商
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