兔抗CD4多克隆
MyBioSource
目录: MBS551067
兔 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 酶免疫法
规格: 0.1毫克
价格: 280美元
至产商
Anti-CD3 epsilon antibody
MyBioSource
目录: MBS355170
兔 多克隆
反应物种: 人类, 小鼠,
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 0.1毫克
价格: 285美元
至产商
兔 单克隆 ([EED-3])
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪, 流式细胞仪

Western blot analysis of CD3 epsilon expression in Jurkat cell lysate (MBS179879). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3E monoclonal antibody overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD3E.

IHC analysis of CD3 epsilon using anti- CD3 epsilon antibody (MBS179879) on human colon cancer. CD3 epsilon was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- CD3 epsilon Antibody (MBS179879) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (MBS176451) with DAB as the chromogen.
规格: 0.03毫克
价格: 160美元
至产商
CD3e(T细胞标记)
MyBioSource
目录: MBS438059
兔 多克隆
反应物种: 人类, 小鼠, 大鼠, , , , 黑猩猩, 猕猴
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 流式细胞仪
CD3(T细胞标记)小鼠单克隆抗体
MyBioSource
目录: MBS439364
小鼠 单克隆 ([RIV9])
反应物种: 人类, 小鼠, 大鼠
应用: 免疫细胞化学, 流式细胞仪, 流式细胞仪