抗体

Rabbit Cortactin (Phospho-Tyr421) Antibody

MBS002638

0.1毫升

440美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 酶免疫法

抗体

Rabbit Cortactin (Phospho-Tyr421) Antibody

[Cortactin (Phospho-Tyr421)]

[src substrate cortactin isoform c; Src substrate cortactin; src substrate cortactin; amplaxin; 1110020L01Rik; oncogene EMS1; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate); cortactin; Amplaxin; Oncogene EMS1]

[CTTN;CTTN;EMS1;EMS1]

SRC8_HUMAN

多克隆

兔

小鼠, 大鼠, 人类

634

Phospho-Cortactin (Tyr421) Ab detects endogenous levels ofCortactin only when phosphorylated at Tyrosine 421

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and nonphospho- peptide affinity columns.

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol

1毫克/毫升

Store at -20°C.Stable for 12 months from date of receipt

酶联免疫吸附测定(EIA), 免疫印迹(免疫印迹)

For western blot, incubate membrane with diluted Ab in 5%w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight

免疫印迹(免疫印迹)

免疫印迹(免疫印迹)

Immunogen: A synthesized peptide derived from human Cortactin aroundthe phosphorylation site of Tyrosine 421. Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mMNaCl, 0.02% sodium azide and 50% glycerol.

Subcellular location: Cytoplasm > cytoskeleton. Cell projection > lamellipodium.Cell projection > ruffle. Associated with membrane rufflesand lamellipodia. Similarity: The SH3 motif may mediate binding to the cytoskeleton.

cortactin a cytoskeletal protein that that is involved incoordinating actin reorganization during cell movement.Localizes at the leading edge of lamellipodia during cellmigration. Its amino-terminal acidic domain associates withthe Arp2/3 and WASP complex at F-actin branches.

296080746

NP_001171669.1

NM_001184740.1

Q14247

85 kDa

Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); E-cadherin Signaling In The Nascent Adherens Junction Pathway (138005); FGF Signaling Pathway (137989); N-cadherin Signaling Events Pathway (137919); Pathogenic Escherichia Coli Infection Pathway (83103); Pathogenic Escherichia Coli Infection Pathway (672459); Pathogenic Escherichia Coli Infection Pathway (516); Proteoglycans In Cancer Pathway (782000); Proteoglycans In Cancer Pathway (782054)

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]

Cortactin: a cytoskeletal protein that that is involved in coordinating actin reorganization during cell movement. Localizes at the leading edge of lamellipodia during cell migration. Its amino-terminal acidic domain associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3. Cortactin may play a role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network. It is degraded in a caspase-dependent manner during apoptosis. It is overexpressed in numerous cancers with poor patient prognosis. It may contribute to tumor cell invasion and metastasis. Two splice variant isoforms have been identified. Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Actin-binding. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: cortical cytoskeleton; voltage-gated potassium channel complex; focal adhesion; dendritic spine; coated pit; actin filament; cell cortex; ruffle; growth cone; cytoskeleton; lamellipodium; cytoplasm; podosome. Molecular Function: protein binding. Biological Process: focal adhesion formation; receptor-mediated endocytosis; intracellular protein transport; positive regulation of actin filament polymerization; actin filament polymerization; actin cytoskeleton reorganization; cell motility involved in cell locomotion; regulation of axon extension; substrate-bound cell migration, cell extension; neurite morphogenesis

0.1毫升

440美元

0.2毫升

715