抗体

兔VE钙粘蛋白(磷酸化Tyr731)抗体

MBS003855

0.1毫升

440美元

多克隆

兔

未共轭

人类, 小鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 酶免疫法

抗体

兔VE钙粘蛋白(磷酸化Tyr731)抗体

[VE钙粘蛋白(磷酸化Tyr731)]

[cadherin-5 preproprotein; Cadherin-5; cadherin-5; 7B4 antigen; VE-cadherin; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium); cadherin 5, type 2 (vascular endothelium); 7B4 antigen; Vascular endothelial cadherin]

[血管内皮钙粘蛋白;血管内皮钙粘蛋白;7B4;CD144;VE钙粘蛋白]

CADH5_HUMAN

多克隆

兔

Human (Identities = 100%, Positives = 100%). Mouse (Identities = 92%, Positives = 100%)

784

VE-Cadherin (Phospho-Tyr731) antibody detects endogenous levels of VE-Cadherin only when phosphorylated at tyrosine 731.

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol

Store at -20 degree C/1 year

酶联免疫吸附测定(EIA), 免疫组化(免疫组化), 免疫印迹(免疫印迹)

WB: 1:500~1:1000。 IHC: 1:50~1:100。 ELISA: 1:20000

试验数据

All Sites: Human: Tyr731; Mouse: Tyr731. Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human VE-Cadherin around the phosphorylation site of tyrosine 731 (H-I-YP-G-Y).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

166362713

NP_001786.2

NM_001795.3

P33151

Adherens Junctions Interactions Pathway (119533); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); S1P2 Pathway (138059); Signaling Events Mediated By VEGFR1 And VEGFR2 Pathway (137940)

This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain. Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral. Chromosomal Location of Human Ortholog: 16q22.1. Cellular Component: tight junction; adherens junction; membrane; plasma membrane; integral to membrane; intercellular junction; cell junction. Molecular Function: protein binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding. Biological Process: intercellular junction assembly and maintenance; negative regulation of cell proliferation; homophilic cell adhesion; vascular endothelial growth factor receptor signaling pathway; blood vessel maturation

0.1毫升

440美元

0.2毫升

715