ELISA/assay

Rat 1,25-Dihydroxy Vitamin D3 ELISA Kit

MBS014354

48-Strip-Wells

435美元

酶联免疫吸附试剂盒

Rat 1,25-Dihydroxy Vitamin D3 ELISA Kit

1,25-Dihydroxy Vitamin D3

1, 25-DVD3

大鼠

No significant cross-reactivity or interference between Rat 1,25-DVD3 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated 1,25-DVD3 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat 1,25-DVD3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 1,25-DVD3 and 1,25-DVD3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 1,25-DVD3 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated 1,25-DVD3 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat 1,25-DVD3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 1,25-DVD3 and 1,25-DVD3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 1,25-DVD3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

48-Strip-Wells

435美元

96-Strip-Wells

600