ELISA/assay

猪5脂肪氧化酶酶联免疫吸附试剂盒

MBS019597

48-Strip-Wells

435美元

酶联免疫吸附试剂盒

猪5脂肪氧化酶酶联免疫吸附试剂盒

5脂肪氧化酶

5-lipoxygenase; Arachidonate 5-lipoxygenase; arachidonate 5-lipoxygenase; LOX-5; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonate 5-lipoxygenase

5-LO

ALOX5;ALOX5;5-LO;5LPG;LOG5;5-LOX;LOG5;5-LO

LOX5_HUMAN

猪

No significant cross-reactivity or interference between Porcine 5-LO and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 U/L - 1000 U/L. Sensitivity: 5.0 U/L.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated 5-LO concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine 5-LO antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 5-LO and 5-LO antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 5-LO in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated 5-LO concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine 5-LO antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 5-LO and 5-LO antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 5-LO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

187167

AAA63212.1

P09917

77,983 Da

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198888); IL-5 Signaling Pathway (198770); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Ovarian Steroidogenesis Pathway (790011); Ovarian Steroidogenesis Pathway (795173); Selenium Pathway (198825)

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer. Protein type: EC 1.13.11.34; Lipid Metabolism - arachidonic acid; Nuclear envelope; Lipid Metabolism - linoleic acid; Oxidoreductase. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: extracellular space; nuclear membrane; nuclear matrix; nuclear envelope lumen; nuclear envelope; cytosol. Molecular Function: protein binding; iron ion binding; arachidonate 5-lipoxygenase activity. Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process. Disease: Asthma, Susceptibility To

48-Strip-Wells

435美元

96-Strip-Wells

600