ELISA/assay

大鼠葡萄糖转运蛋白2酶联免疫吸附试剂盒

MBS020970

48-Strip-Wells

435美元

酶联免疫吸附试剂盒

大鼠葡萄糖转运蛋白2酶联免疫吸附试剂盒

葡萄糖转运蛋白2

GLUT2; Solute carrier family 2, facilitated glucose transporter member 2; solute carrier family 2, facilitated glucose transporter member 2; liver-type glucose transporter; glucose transporter type 2, liver; solute carrier family 2 (facilitated glucose transporter), member 2; Glucose transporter type 2, liver

GLUT2

Slc2a2; Slc2a2; Glut2; Glut-2; AI266973; Glut-2; Glut2; GLUT-2

GTR2_MOUSE

大鼠

No significant cross-reactivity or interference between Rat GLUT2 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GLUT2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GLUT2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GLUT2 and GLUT2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GLUT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GLUT2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GLUT2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GLUT2 and GLUT2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GLUT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

683589

CAA55368.1

P14246

57,107 Da

Carbohydrate Digestion And Absorption Pathway (170722); Carbohydrate Digestion And Absorption Pathway (170654); Developmental Biology Pathway (820229); Facilitative Na+-independent Glucose Transporters Pathway (819170); Glucose Transport Pathway (819167); Glycolysis And Gluconeogenesis Pathway (198376); Hexose Transport Pathway (819166); Insulin Secretion Pathway (777543); Integration Of Energy Metabolism Pathway (819329); Maturity Onset Diabetes Of The Young Pathway (83293)

GLUT2: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Present at high levels in the plasma membrane of pancreatic b-cells where it initiates the first step in glucose-stimulated insulin secretion, a glucose metabolism-dependent signaling mechanism. May mediate the bidirectional transfer of glucose across the plasma membrane of hepatocytes. May also participate with the Na( +2)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Required for glucose sensitivity in the hypothalamus and brain stem. Involved in the control of food intake and stimulation of glucose uptake by peripheral tissues. Protein type: Transporter; Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass. Cellular Component: membrane; basolateral plasma membrane; brush border membrane; cytoplasm; integral to membrane; plasma membrane; cytosol; endosome; brush border. Molecular Function: D-glucose transmembrane transporter activity; dehydroascorbic acid transporter activity; transporter activity; glucose transmembrane transporter activity; transmembrane transporter activity; substrate-specific transmembrane transporter activity; insulin receptor binding. Biological Process: carbohydrate utilization; transport; carbohydrate transport; glucose transport; transmembrane transport

48-Strip-Wells

435美元

96-Strip-Wells

600