ELISA/assay

Monkey Tissue Factor ELISA Kit

MBS046786

48-Strip-Wells

435美元

酶联免疫吸附试剂盒

Monkey Tissue Factor ELISA Kit

Tissue Factor

tissue factor; Tissue factor; tissue factor; coagulation factor III (thromboplastin, tissue factor); Coagulation factor III; Thromboplastin

TF

F3;F3;TF;TFA;CD142;TF

TF_HUMAN

Monkey

No significant cross-reactivity or interference between Monkey TF and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 15.6 pg/ml - 500 pg/ml. Sensitivity: 2.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TF concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey TF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TF and TF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TF in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TF concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey TF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TF and TF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

339508

AAA36734.1

P13726

33,068 Da

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Extrinsic Pathway (106058); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028)

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

F3: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. TF expression is highly dependent upon cell type. TF can also be induced by the inflammatory mediators interleukin 1 and TNF-alpha, as well as by endotoxin, to appear on monocytes and vascular endothelial cells as a component of cellular immune response. Lung, placenta and pancreas. Belongs to the tissue factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 1p22-p21. Cellular Component: extracellular matrix; extracellular space; cell surface; integral to membrane; plasma membrane. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protease binding; phospholipid binding. Biological Process: caspase activation; positive regulation of protein kinase B signaling cascade; activation of blood coagulation via clotting cascade; blood coagulation, extrinsic pathway; positive regulation of angiogenesis; activation of plasma proteins during acute inflammatory response; positive regulation of positive chemotaxis; cytokine and chemokine mediated signaling pathway; positive regulation of endothelial cell proliferation; blood coagulation; positive regulation of cell migration

48-Strip-Wells

435美元

96-Strip-Wells

600