ELISA/assay

Bovine Fibroblast Growth Factor 2, Basic ELISA Kit

MBS096912

48-Strip-Wells

435美元

酶联免疫吸附试剂盒

Bovine Fibroblast Growth Factor 2, Basic ELISA Kit

Fibroblast Growth Factor 2, Basic

fibroblast growth factor 2; Fibroblast growth factor 2; fibroblast growth factor 2; prostatropin; heparin-binding growth factor 2; basic fibroblast growth factor bFGF; fibroblast growth factor 2 (basic); Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2

纤维生长因子2

纤维生长因子2;纤维生长因子2;BFGF;FGFB;FGF-2;HBGF-2;FGFB;FGF-2;bFGF;HBGF-2

FGF2_HUMAN

牛

288

No significant cross-reactivity or interference between Bovine FGF2 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 pg/ml - 200 pg/ml. Sensitivity: 1.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated FGF2 concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine FGF2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FGF2 and FGF2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FGF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated FGF2 concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine FGF2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FGF2 and FGF2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FGF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

153285461

NP_001997.5

NM_002006.4

P09038

30,770 Da

Activated Point Mutants Of FGFR2 Pathway (645281); Adaptive Immune System Pathway (366160); Angiogenesis Pathway (198772); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Cardiac Progenitor Differentiation Pathway (712094); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Developmental Biology Pathway (477129); Disease Pathway (530764)

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]

FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation. Protein type: Activator; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4q26. Cellular Component: extracellular space; extracellular region; nucleus. Molecular Function: heparin binding; protein binding; growth factor activity; ligand-dependent nuclear receptor transcription coactivator activity; cytokine activity; chemoattractant activity; fibroblast growth factor receptor binding. Biological Process: extracellular matrix organization and biogenesis; wound healing; nerve growth factor receptor signaling pathway; somatic stem cell maintenance; activation of MAPK activity; positive regulation of transcription, DNA-dependent; chemotaxis; signal transduction; hyaluronan catabolic process; growth factor dependent regulation of satellite cell proliferation; positive regulation of MAP kinase activity; positive chemotaxis; positive regulation of cell proliferation; embryonic morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of cell fate specification; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; negative regulation of blood vessel endothelial cell migration; regulation of angiogenesis; positive regulation of angiogenesis; organ morphogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; positive regulation of cell division; release of sequestered calcium ion into cytosol; Ras protein signal transduction; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; inositol phosphate biosynthetic process

48-Strip-Wells

435美元

96-Strip-Wells

600