抗体

小鼠抗人类弹性蛋白

MBS140123

0.5毫克

305美元

单克隆

小鼠

未共轭

hVimentin

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 酶免疫法

抗体

小鼠抗人类弹性蛋白

弹性蛋白

弹性蛋白抗体;弹性蛋白, 小鼠抗人类抗体;弹性蛋白

弹性蛋白;弹性蛋白;弹性蛋白;附睾苯巴比妥蛋白113;弹性蛋白

弹性蛋白;弹性蛋白;HEL113;CTRCT30

VIME_HUMAN

单克隆

IgG1

hVimentin

小鼠

Vimentin antibody will recognize most mammalian Vimentin except mouse and rat.

466

Ion exchange column.

1mg/ml in PBS (after reconstitution).

In lyophilized form, for long periods, store at 4 degree C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20 degree C. Antibody is shipped lyophilized at ambient temperature.

酶联免疫吸附测定(EIA), 免疫印迹(免疫印迹), 免疫沉淀(免疫沉淀), 免疫组化(免疫组化)

Direct ELISA, Western Blot, Immunoprecipitation, immunohistochemistry. For intra-cellular staining, use 5-10 ul/1,000,000 cells. Wash twice with PBS/Azide before adding the appropriate amount of secondary fluorescent reagent.

Other Available Forms: This antibody is also available conjugated to biotin and FITC.

Reconstitution: Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Immunogen: Purified Human Vimentin.

单克隆/多克隆ANTIBODIES, MISCELLANEOUS ANTIBODIES;单克隆抗体

Introduction: Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines.

62414289

NP_003371.2

NM_003380.3

53,652 Da

Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); Aurora B Signaling Pathway (138080); Caspase Cascade In Apoptosis Pathway (137974); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (105679); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); MicroRNAs In Cancer Pathway (852705)

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue. Protein type: Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 10p13. Cellular Component: intermediate filament cytoskeleton; neuron projection; cytoskeleton; focal adhesion; leading edge; cytoplasm; plasma membrane; intermediate filament; peroxisome; cytosol. Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens. Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; muscle filament sliding; cell structure disassembly during apoptosis. Disease: Cataract 30

0.5毫克

305美元

1毫克

455