产品简要
公司名称 :
MyBioSource
产品类型 :
蛋白
产品名称 :
Recombinant Human Stromal Cell-Derived Factor-1 beta (CXCL12)
目录 :
MBS142032
规格 :
0.01毫克
价格 :
205美元
更多信息或购买 :
产品信息
目录号 :
MBS142032
产品类型 :
重组蛋白
产品全称 :
Recombinant Human Stromal Cell-Derived Factor-1 beta (CXCL12)
产品简称 :
Stromal Cell-Derived Factor-1 beta
产品名称同义词 :
SDF 1b Human; Stromal Cell Derived Factor-1 Beta Human Recombinant (CXCL12); SDF-1; CXCL12; Pre-B cell growth-stimulating factor; PBSF; hIRH; chemokine (C-X-C motif) ligand 12; SDF1; SDF1B; TPAR1; SCYB12; SDF-1b; TLSF-b
其他名称 :
stromal cell-derived factor 1 isoform beta; Stromal cell-derived factor 1; stromal cell-derived factor 1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor; chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor; PBSF
产品基因名称 :
SDF 1b
其他基因名称 :
CXCL12;CXCL12;IRH;PBSF;SDF1;TLSF;TPAR1;SCYB12;SDF1;SDF1A;SDF1B;SDF-1;hSDF-1;IRH;hIRH;PBSF
UniProt数据库进入名 :
SDF1_HUMAN
宿主 :
大肠杆菌
序列长度 :
93
序列 :
The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu.
纯度 :
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
形式 :
The CXCL12 protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. Sterile Filtered White lyophilized (freeze-dried) powder.
储存稳定性 :
Lyophilized SDF-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CXCL12 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
其他信息2 :
Solubility: It is recommended to reconstitute the lyophilized Stromal Cell-Derived Factor-1b in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of SDF-1b as a Reference Standard. Biological Activity: The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80ng/ml corresponding to a Specific Activity of 12,500-50,000IU/mg.
产品种类 :
CHEMOKINES;趋化因子;SDF(CXCL12)
产品描述 :
Description: Stromal Cell-Derived Factor-1 beta Human Recombinant produced in E Coli is a non-glycosylated, Polypeptide chain containing 72 amino acids and having a molecular mass of 8508 Dalton. The SDF-1b is purified by proprietary chromatographic techniques. Introduction: SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). It is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice (including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow).The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive (unlike for other chemokines and their receptors), but recently it was suggested that CXCL12 is also bound by CXCR7 receptor. The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.
NCBI GI登录号 :
10834988
NCBI登录号 :
NP_000600.1
NCBI基因登录号 :
NM_000609.6
UniProt数据库登录号 :
P48061
NCBI分子量 :
11,004 Da
NCBI信号通路 :
Axon Guidance Pathway 83065!!Axon Guidance Pathway 476!!CXCR4-mediated Signaling Events Pathway 137910!!Chemokine Receptors Bind Chemokines Pathway 106359!!Chemokine Signaling Pathway 99051!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway 1127664
NCBI总结 :
This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
规格 :
0.01毫克
价格 :
205美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。