产品简要
公司名称 :
MyBioSource
产品类型 :
蛋白
产品名称 :
Recombinant Pfu DNA Polymerase
目录 :
MBS142406
规格 :
100单位
价格 :
205美元
更多信息或购买 :
产品信息
目录号 :
MBS142406
产品类型 :
重组蛋白
产品全称 :
Recombinant Pfu DNA Polymerase
产品简称 :
Pfu DNA Polymerase
产品名称同义词 :
Pfu DNA Polymerase; Pfu-DNA Polymerase Recombinant; DNA polymerase; EC 2.7.7.7; Pfu polymerase; Pfu-DNA Polymerase; Pfu DNA Polymerase
其他名称 :
DNA-directed DNA polymerase; DNA polymerase; DNA-directed DNA polymerase; DNA polymerase B; Pfu polymerase; Pol I
其他基因名称 :
PF0212; pol
UniProt数据库进入名 :
DPOL_PYRFU
宿主 :
大肠杆菌
序列长度 :
775
形式 :
50mM Tris-HCl, pH 8.2, 1mM DTT, 0.1mM EDTA, 0.05% CHAPS and 50% glycerol. Sterile liquid formulation.
储存稳定性 :
Pfu DNA Polymerase although stable at 10 degree C for 5 days, should be stored below -18 degree C.Please prevent freeze-thaw cycles.
应用笔记 :
1. Ideal for high-fidelity amplification. 2. 3'-5' exonuclease activity provides a low error rate. 3. One of the most thermostable DNA polymerases known. 4. Lack of extendase activity means no unwanted 3' overhangs. 5. Optimal for blunt-end PCR cloning. 6. Optimum temperature near 75 degree C. 7. 95% active after 1-hour incubation at 98 degree C.
其它信息1 :
10X Buffer with MgSO4: 200mM Tris-HCl (pH 8.8 at 25 degree C), 100mM (NH4)2SO4, 100mM KC1, 1% Triton X-100, 1mg/ml BSA, 20mM MgSO4. PCR Protocol: Add the following components to a amplify 1kb DNA template: 5 ul 10x buffer with MgSO4. 4 ul 2.5mM dNTPs. 1 ul Primers mix (10 uM each). 0.2 ul Pfu-DNA Polymerase. and 38 ul Water. Amplify using the following cycling parameters: Heat Soak: 1 cycle at 94 degree C/4 min. Denaturation: 30 cycles at 94 degree C/30 sec. Annealing: 30 cycles at 58 degree C /30 sec. Extension: 30 cycles at 72 degree C /90 sec. Final: 1 cycle at 72 degree C /5 min. Unit Defenition: 1U of enzyme catalyzes the incorporation of 10nmol of dNTP into acid-insoluble product in 30 minutes at 75 degree C.
产品种类 :
ENZYMES;酶;DNA聚合酶
产品描述 :
Description: Pfu DNA Polymerase is a thermo-stable enzyme having a Mw of about 90kDa. Pfu DNA Polymerase is derived from E Coli that and cloned from Pyrococcus furiosus strain Vc1 DSM3638. Pfu DNA Polymerase replicates DNA at 75 degree C, catalyzing the polymerization of nucleotides into duplex DNA in the 5 ´ to 3 ´ direction in the existence of magnesium. Pfu DNA Polymerase possesses 3 ´ to 5 ´ exonuclease (proofreading) activity. Base misinsertions that take place during polymerization are swiftly removed by the proofreading activity of the polymerase. Therefore, Pfu DNA Polymerase is suggested for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended. Introduction: Pfu DNA polymeraseenzyme is found in the hyperthermophilic archaeonPyrococcus furiosus, where it functions in vivoto replicate the organism's DNA. In vitro, Pfu is used to swiftly amplify DNAin the Polymerase Chain Reaction, where the enzyme serves the central function of copying a new strand of DNA during each extension step. Pfu DNA polymerase has superior thermostability and 'proofreading' properties compared to other thermostable polymerases. Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3' to 5' exonuclease proof reading activity, meaning that it works its way along the DNA from the 5' endto the 3' endand corrects nucleotidemisin corporation errors. Pfu DNA polymerase-generated PCRfragments will have fewer errors than Taq-generated PCR inserts. As a result, Pfu is more commonly used for molecular cloning of PCR fragments than the historically popular Taq. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity.
NCBI GI登录号 :
18976584
NCBI登录号 :
NP_577941.1
NCBI基因登录号 :
NC_003413.1
UniProt数据库登录号 :
P61875
NCBI分子量 :
90,113 Da
NCBI信号通路 :
Metabolic Pathways (132066); Purine Metabolism Pathway (7520); Purine Metabolism Pathway (307); Pyrimidine Metabolism Pathway (7521); Pyrimidine Metabolism Pathway (309)
尺寸1 :
100单位
价格1 :
205美元
尺寸2 :
500单位
价格2 :
290
size3 :
2500单位
价格3 :
965
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。