产品简要
公司名称 :
MyBioSource
产品类型 :
蛋白
产品名称 :
重组Taq DNA聚合酶
目录 :
MBS142449
规格 :
1000单位
价格 :
140美元
更多信息或购买 :
产品信息
目录号 :
MBS142449
产品类型 :
重组蛋白
产品全称 :
重组Taq DNA聚合酶
产品简称 :
Taq DNA聚合酶
产品名称同义词 :
TaqDNA; Taq DNA Polymerase Recombinant; DNA polymerase I thermostable; EC 2.7.7.7; Taq polymerase 1; TaqDNA
其他名称 :
DNA polymerase I, thermostable; DNA polymerase I, thermostable; Taq polymerase 1
其他基因名称 :
polA; pol1
UniProt数据库进入名 :
DPO1_THEAQ
宿主 :
重组大肠杆菌含Taq聚合酶基因
序列长度 :
832
形式 :
Taq DNA聚合酶溶液在20mM三羟甲基氨基甲烷氯化氢, pH值8.0, 100mM氯化钾, 0.1mM乙二胺四乙酸, 5mM DTT, 50% 甘油, 0.5% NP40, 0.5% 吐温20
储存稳定性 :
Stable for 5 days at 10 degree C, for longer period of time store at -20 degree C.
其它信息1 :
Include: : 10X Reaction Buffer without MgCl2 and separate 25mM MgCl2 Solution. Features: Depend on an Enzyme That Works: Compositions of the storage buffers have been optimized to assure quality performance of the enzyme under a variety of conditions. Specify Your Own Reaction ConditionsChoose either Taq with Mg-free 10X Reaction Buffer and separate 25mM MgCl2 or Taq with 10X Reaction Buffer containing 15mM MgCl2. Rely on a Performance-Tested EnzymeOur PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any of our PCR product, we will send a replacement or refund your account. Unit Defenition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74 degree C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25 degree C), 50mM NaCl, 5mM MgCl2, 200um each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10?g activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul. 10X Reaction Buffer with MgCl2500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C), 1% Triton X-100 and 15mM MgCl2. Buffer is optimized for use with 0.2mM of each dNTP. 10X Reaction Buffer without MgCl2500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C) and 1% Triton X-100. Storage Buffer: Compatibility with Reaction Buffers: Taq DNA Polymerase in Storage Buffer. Use of other reaction buffers that do not contain Triton X-100 (final concentration of 0.1%) will result in inactivation of the enzyme. 50mM Tris-HCl (pH 8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol and 1% Triton X-100.
其他信息2 :
QC Test: PCR, activity, SDS-PAGE/purity, endonuclease/nickase.
产品种类 :
ENZYMES;酶;DNA聚合酶
产品描述 :
Description: Taq DNA Polymerase(a) is a thermostable enzyme of approximately 94 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74 degree C and exhibits a half-life of 40 minutes at 95 degree C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5 ´~3 ´ direction in the presence of magnesium and also possesses a 5 ´~3 ´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
NCBI GI登录号 :
118828
NCBI登录号 :
P19821.1
UniProt数据库登录号 :
P19821
NCBI分子量 :
93,910 Da
尺寸1 :
1000单位
价格1 :
140美元
尺寸2 :
3000单位
价格2 :
205
size3 :
10000单位
价格3 :
415
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。