蛋白

Recombinant Taq Plus DNA Polymerase

MBS142450

250单位

140美元

重组蛋白

Recombinant Taq Plus DNA Polymerase

Taq Plus DNA Polymerase

Taq Plus DNA; Taq Plus DNA Polymerase Recombinant; Taq Plus DNA Polymerase; Taq Plus DNA; TaqPDNA; Taq Plus DNA

重组大肠杆菌含Taq聚合酶基因

5U/微升

Stable for 5 days at 10 degree C, for longer period of time store at -20 degree C.

10X Reaction Buffer: 200 mM TrisHCI (pH 8.8)100 mM KCI100 mM (NH4)2SO420 mM Mg SO41% Triton X-1001 mg/ml bovine serum albumin (BSA). Optimization of DNA Synthesis: It is important to ad the reaction components in the following order1. H2O.2. 10 x reaction buffer.3. dNTPs.4. DNA template and primers.5. Taq Plus. Reaction Conditions: DNA synthesis is performed in 100ml of mixture containing 20-200um dNTPs, 0.3-1um Promers, 0.1-0.250 mg of template DNA, 10 ?l of 10 x reaction buffer and 2.5-5 units of Taq Plus. Mix the reaction gently, centrifuge briefly and then overlay with light mineral oil. Initially, denature the reaction by incubating at 95? for 5 minutes and then cool to 40-68? for 5 minutes to allow the primers to anneal to the template DNA. Unit Defenition: One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30 min at 74 degree C. Storage Buffer: 20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, Stabilizers, and 50%glycerol.

Note: All reagents, including Taq Plus, should be mixed immediately before use.

ENZYMES;酶;DNA聚合酶

Description: Taq Plus is a mixture of Taq and Pfu. Taq Plus, which is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages over Taq: (1) high fidelity with an error frequency 1.6/106 (or 0.0016/103) during DNA synthesis. (2) Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb. Pfu has a temperature optimum between 72-78 degree C and remains > 95% active following 1-hour incubation at 95 degree C.

250单位

140美元

750单位

205

2500单位

415