免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 酶免疫法
TOCA-1; TOCA1; C1orf39; TOCA1; Formin-binding protein 1-like; Transducer of Cdc42-dependent actin assembly protein 1; Toca-1; formin binding protein 1-like
formin-binding protein 1-like isoform 1; Formin-binding protein 1-like; formin-binding protein 1-like; toca-1; transducer of Cdc42-dependent actin assembly 1; transducer of Cdc42-dependent actin assembly protein 1; formin binding protein 1-like; Transducer of Cdc42-dependent actin assembly protein 1; Toca-1
FNBP1L; FNBP1L; TOCA1; C1orf39; C1orf39; TOCA1; Toca-1
人类, 小鼠, 大鼠
TOCA-1 Antibody is affinity chromatography purified via peptide column.
TOCA-1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
酶联免疫吸附测定(EIA), 免疫印迹(免疫印迹), 免疫组化(免疫组化), 免疫荧光(IF)
TOCA-1 antibody can be used for detection of TOCA-1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate: Unconjugated. Immunogen: TOCA-1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human TOCA-1. Buffer: TOCA-1 Antibody is supplied in PBS containing 0.02% sodium azide.
TOCA-1 Antibody: Actin reorganization is important for the regulation of neuronal morphology. A protein involved in this process, the transducer of cdc42-dependent actin assembly 1 (TOCA-1) protein, a member of the evolutionarily conserved pombe CDC15 homology (PCH) protein family, is an essential component of the Cdc42 pathway. TOCA-1 binds both N-WASP and Cdc42 and is essential for Cdc42- and PIP2-induced actin polymerization, suggesting that TOCA-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Decreased expression of TOCA-1 significantly enhances neurite elongation in PC-12 cells; its overexpression in the same cells suppresses neurite elongation. It has been suggested that TOCA-1 negatively regulates axon branching by regulating membrane trafficking by regulating membrane trafficking through the F-BAR/EFC domain. Multiple isoforms of TOCA-1 are known to exist.
NCBI GI登录号 :
The protein encoded by this gene binds to both CDC42 and N-WASP. This protein promotes CDC42-induced actin polymerization by activating the N-WASP-WIP complex and, therefore, is involved in a pathway that links cell surface signals to the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
FNBP1L: Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Essential for autophagy of intracellular bacterial pathogens. Belongs to the FNBP1 family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 1p22.1. Cellular Component: cytoskeleton; cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; cell cortex. Molecular Function: protein binding; lipid binding. Biological Process: positive regulation of filopodium formation; vesicle transport along actin filament; vesicle organization and biogenesis; membrane invagination; cilium biogenesis; autophagy; membrane budding
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San Diego, CA 92195-3308
San Diego, CA 92195-3308