ELISA/assay

Human forkhead box P3, Foxp3 ELISA Kit

MBS162054

48-Strip-Wells

260美元

酶联免疫吸附试剂盒

Human forkhead box P3, Foxp3 ELISA Kit

[forkhead box P3, Foxp3]

[forkhead box protein P3 isoform b; Forkhead box protein P3; forkhead box protein P3; forkhead box P3; Scurfin]

[Foxp3]

[FOXP3;FOXP3;JM2;AIID;IPEX;PIDX;XPID;DIETER;IPEX]

人类

396

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.2ng/ml - 60ng/ml. Sensitivity: 0.09ng/ml

Intra-Assay: CV<8% . Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Forkhead Box P3 (also known as Foxp3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human Foxp3 antibody. Foxp3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human Foxp3 Antibody is added and binds to Foxp3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Foxp3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human Foxp3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

167466190

NP_001107849.1

NM_001114377.1

Q9BZS1

43,410 Da

Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); IL2 Signaling Events Mediated By STAT5 Pathway (137988); Inflammatory Bowel Disease Pathway (842771)

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 ().

48-Strip-Wells

260美元

96-Strip-Wells

395

5x96-Strip-Wells

1610

10x96-Strip-Wells

3135