产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
Anti-TSH Receptor Picoband Antibody
目录 :
MBS1750438
规格 :
0.1毫克
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
人类, 大鼠
应用 :
免疫印迹
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS1750438 图像 1
Figure 1. Western blot analysis of TSH Receptor using anti-TSH Receptor antibody (MBS1750438). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSH Receptor antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TSH Receptor at approximately 86KD. The expected band size for TSH Receptor is at 86KD.
产品信息
目录号 :
MBS1750438
产品类型 :
抗体
产品全称 :
Anti-TSH Receptor Picoband Antibody
产品简称 :
[TSH受体]
产品名称同义词 :
[促甲状腺激素受体;甲状腺刺激激素受体;TSH-R;TSHR;LGR3]
其他名称 :
[thyrotropin receptor isoform 1; Thyrotropin receptor; thyrotropin receptor; thyroid stimulating hormone receptor; Thyroid-stimulating hormone receptor; TSH-R]
产品基因名称 :
[TSHR]
其他基因名称 :
[TSHR;TSHR;LGR3;CHNG1;hTSHR-I;LGR3;TSH-R]
克隆性 :
多克隆
抗体亚型 :
IgG
宿主 :
反应物种 :
Reacts with: Mouse, Rat. Predicted to work with: Human
序列长度 :
274
特异性 :
No cross reactivity with other proteins.
纯度 :
免疫原亲和纯化
形式 :
冻干
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
储存稳定性 :
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
检测过的应用 :
免疫印迹(免疫印迹)
应用笔记 :
WB: 0.1-0.5ug/ml. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
image1头 :
免疫印迹(免疫印迹)
其它信息1 :
Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human TSH Receptor (244-277aa KGLEHLKELIARNTWTLKKLPLSLSFLHLTRADL), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid. Subcellular Localization: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in the thyroid.
其他信息2 :
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
产品种类 :
信号转导;生长因子/激素;神经科学;内分泌系统;甲状腺枢椎
产品描述 :
Description: TSHR (Thyroid-Stimulating Hormone Receptor), also called LGR3, is mapped to 14q31.1. The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene.
NCBI GI登录号 :
64085121
NCBI登录号 :
NP_000360.2
NCBI基因登录号 :
NM_000369.2
UniProt数据库登录号 :
P16473
NCBI分子量 :
86830分子量
NCBI信号通路 :
Arf6 Signaling Events Pathway (138034); Arf6 Trafficking Events Pathway (137954); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Hormone Ligand-binding Receptors Pathway (106369); Neuroactive Ligand-receptor Interaction Pathway (83053)
NCBI总结 :
The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
UniProt数据库总结 :
Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin (PubMed:11847099, PubMed:12045258). Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin (PubMed:12045258). The activity of this receptor is mediated by G proteins which activate adenylate cyclase (PubMed:11847099). Plays a central role in controlling thyroid cell metabolism ().
尺寸1 :
0.1毫克
价格1 :
280美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。