ELISA/assay

Mouse NGF/NGF beta PicoKine ELISA Kit

MBS175827

96 Wells

360美元

酶联免疫吸附试剂盒

Mouse NGF/NGF beta PicoKine ELISA Kit

神经生长因子/神经生长因子乙

Beta-nerve growth factor; nerve growth factor (beta polypeptide); Beta nerve growth factor; Beta NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor (beta polypeptide); Nerve growth factor beta polypeptide; Nerve growth factor beta subunit; NGFB; NID67

β神经生长因子;β神经生长因子;β神经生长因子;乙神经生长因子;神经生长因子, 乙;神经生长因子

神经生长因子

Ngf; Ngf; Ngfb; Ngfb; Beta-NGF

NGF_MOUSE

小鼠

241

Natura! and recombinant mouse NGF

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates and serum. Assay Type: Sandwich. Detection Range: 31.2pg/ml-2000pg/ml. Sensitivity: < 1 pg/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the Assay: The mouse NGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for NGF has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: S122-G241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse NGF amount of sample captured in plate.

128162

P01139.2

P01139

27,077 Da

ARMS-mediated Activation Pathway (970820); Activation Of TRKA Receptors Pathway (1000905); Apoptosis Pathway (83257); Apoptosis Pathway (470); Apoptosis Signaling Pathway (522973); Axonal Growth Stimulation Pathway (1000902); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (1000892); Ceramide Signalling Pathway (1000900); Frs2-mediated Activation Pathway (971392); Id Signaling Pathway (198411)

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; cytoplasmic membrane-bound vesicle; extracellular region. Molecular Function: metalloendopeptidase inhibitor activity; nerve growth factor receptor binding; growth factor activity; receptor signaling protein activity; receptor binding. Biological Process: positive regulation of neuron maturation; regulation of neuron differentiation; adult locomotory behavior; positive regulation of axon extension; regulation of neurotransmitter secretion; sensory perception of pain; positive regulation of cell growth; positive regulation of protein amino acid autophosphorylation; positive regulation of nerve growth factor receptor signaling pathway; memory; regulation of release of sequestered calcium ion into cytosol; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; positive regulation of cell proliferation; positive regulation of transcription factor activity; negative regulation of neuron apoptosis; positive regulation of neuron differentiation; positive regulation of collateral sprouting; transmembrane receptor protein tyrosine kinase signaling pathway; neurite morphogenesis; neurite development

96 Wells

360美元