ELISA/assay

Rat VEGF PicoKine ELISA Kit

MBS175844

96 Wells

380美元

酶联免疫吸附试剂盒

Rat VEGF PicoKine ELISA Kit

血管内皮生长因子

血管内皮生长因子;血管内皮生长因子;MGC70609;MVCD1;血管内皮生长因子;血管内皮生长因子;血管通透性因子;血管内皮生长因子a;血管内皮生长因子A;VPF

血管内皮生长因子;血管内皮生长因子;血管通透性因子;血管内皮生长因子;血管内皮生长因子

血管内皮生长因子A

血管内皮生长因子A;血管内皮生长因子A;VPF;血管内皮生长因子;血管内皮生长因子-A;VEGF164

B5DEK7_RAT

大鼠

369

Natural and recombinant rat VEGF

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum and plasma(heparin, EDTA, citrate). Assay Type: Quantitative Sandwich. Sensitivity: < 1pg/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the Assay: The rat VEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGF has been precoated onto 96-well plates. Standards (NSQ,A27 -R 190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat VEGF amount of sample captured in plate. Background/Introduction: Vascular permeability factor/va~)cular endothelial growth factor (VPFNEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.vEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias.vEGF may also playa pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.The standard product used in this kit is recombinant rat VEGF164, which is a 25KDa single chain as well as a 50KDa dimer.

B5DEK7

40,241 Da

Axon Guidance Pathway (1009871); Bladder Cancer Pathway (83503); Bladder Cancer Pathway (527); Cellular Response To Hypoxia Pathway (1010375); Cellular Responses To Stress Pathway (1010374); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (1009870); EPH-Ephrin Signaling Pathway (1009905); EPHA-mediated Growth Cone Collapse Pathway (1009906)

This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. The expression of some isoforms derived from AUG start codon is affected by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2013]

VEGF: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the PDGF/VEGF growth factor family. 13 isoforms of the human protein are produced by alternative promoter. Protein type: Secreted; Cytokine; Motility/polarity/chemotaxis; Secreted, signal peptide. Cellular Component: extracellular space; cell surface; membrane; cytoplasm; extracellular region; basement membrane; secretory granule. Molecular Function: heparin binding; identical protein binding; protein homodimerization activity; growth factor activity; extracellular matrix binding; cytokine activity; platelet-derived growth factor receptor binding; vascular endothelial growth factor receptor 1 binding; vascular endothelial growth factor receptor binding; receptor agonist activity; vascular endothelial growth factor receptor 2 binding; protein heterodimerization activity; fibronectin binding; receptor binding; chemoattractant activity. Biological Process: heart morphogenesis; macrophage differentiation; positive regulation of cell adhesion; positive regulation of positive chemotaxis; positive regulation of receptor internalization; cell maturation; female pregnancy; response to vitamin A; basophil chemotaxis; positive regulation of MAP kinase activity; regulation of cell shape; positive chemotaxis; response to folic acid; positive regulation of mesenchymal cell proliferation; mesoderm development; negative regulation of neuron apoptosis; kidney development; positive regulation of neuroblast proliferation; nervous system development; T-helper 1 type immune response; monocyte differentiation; positive regulation of signal transduction; mRNA stabilization; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; positive regulation of protein amino acid autophosphorylation; regulation of endothelial cell differentiation; positive regulation of vascular permeability; regulation of transcription from RNA polymerase II promoter; patterning of blood vessels; eye photoreceptor cell development; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; camera-type eye morphogenesis; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; cardiac muscle fiber development; positive regulation of cell division; activation of protein kinase activity; positive regulation of axon extension involved in axon guidance; neuron development; endothelial cell migration; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of cGMP metabolic process; response to progesterone stimulus; alveolus development; surfactant homeostasis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; lactation; post-embryonic camera-type eye development; wound healing; positive regulation of smooth muscle cell proliferation; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of vascular endothelial growth factor receptor signaling pathway; response to estradiol stimulus; induction of positive chemotaxis; vasculature development; epithelial cell differentiation; ovarian follicle development; positive regulation of focal adhesion formation; lymphangiogenesis; positive regulation of cell proliferation; negative regulation of programmed cell death; angiogenesis; cell differentiation; negative regulation of bone resorption; aging; blood vessel development; cell migration; in utero embryonic development; lumen formation; positive regulation of cell motility; positive regulation of peptidyl-serine phosphorylation; cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of protein complex assembly; response to hypoxia; artery morphogenesis; blood vessel remodeling; response to cold; sprouting angiogenesis; positive regulation of protein amino acid phosphorylation; negative regulation of cell-cell adhesion; vascular endothelial growth factor receptor signaling pathway; hyaluronan metabolic process; lung development; positive regulation of cell migration; growth

96 Wells

380美元